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Biotinylated Nucleotides for DNA Labeling


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

 

Table 1: Enzymatic incorporation of Biotinylated Nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT:: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)


DNA LabelingcDNA
Labeling
NucleotideCat. No.PCR NTPrimer Extension3' End LabelingRT
dUTP
Biotin-11-dUTP
C5 position
NU-803-BIOX1,22222
Biotin-16-dUTP
C5 position
NU-803-BIO161,22222
dCTP
Biotin-11-dCTP
C5 position
NU-809-BIOX1,22222
Biotin-16-dCTP
C5 position
NU-809-BIO1612222
Biotin-14-dCTP
N4 position
NU-956-BIO14-22n/an/a
dATP
Biotin-7-dATP
N6 position
NU-835-BIO-22-n/a
Biotin-14-dATP
N6 position
NU-835-BIO14-22n/an/a
Desthiobiotin-7-dATP
N6 position
NU-835-Desthiobio-n/a2n/an/a
Biotin-11-dATP
7-Deaza position
NU-1175-BIOX1,222n/a2
dGTP
Biotin-11-dGTP
7-Deaza position
NU-1615-BIOXn/an/an/an/an/a
ddUTP
Biotin-11-ddUTP
C5 position
NU-1619-BIOX---2-
ddATP
Biotin-11-ddATP
7-Deaza position
NU-1612-BIOX---2-

1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable