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Biotin-14-N6-(6-Aminohexyl)-dATP, Triethylammonium salt


Cat. No. Amount Price (EUR) Buy / Note
NU-835-BIO14-S 200 μl (1 mM)122,80 Add to Basket/Quote Add to Notepad
NU-835-BIO14-L 5 x 200 μl (1 mM)375,70 Add to Basket/Quote Add to Notepad
Structural formula of Biotin-14-dATP (Biotin-14-N6-(6-Aminohexyl)-dATP, Triethylammonium salt)
Structural formula of Biotin-14-dATP

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C32H54N9O15P3S (free acid)

Molecular Weight: 929.81 g/mol (free acid)

Exact Mass: 929.27 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: filtered solution (30 kDa) in 10 mM Tris-HCl

Color: colorless to slightly yellow

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I [1] & in-house data
- Primer Extension with Klenow fragment [2]

Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taq polymerase Biotin-11-dATP (#NU-1175-BIOX) is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker.

Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/ 50% dATP

Please note: The optimal final concentration of Biotin-14-dATP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-14-dATP/dATP ratio is recommended.

Related products:

BIOZ Product Citations:

Selected References:
[1] Gebeyehu et al. (1987) Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA. Nucleic Acids Res. 15 (21):4513.
[2] Nagano et al. (2015) Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell. Nature Protocols 10 (12):1987.
Mumbach et al. (2016) HiChIP: Efficient and sensitive analysis of protein-directed genome architecture Nature Protocols 13 (11):919.