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Biotin-14-dATP

Biotin-14-N6-(6-Amino)hexyl-dATP, Triethylammonium salt

Bio-14-dATP

Cat. No. Amount Price (EUR) Buy / Note
NU-835-BIO14-S 200 μl (1 mM) 92,25 Add to Basket/Quote Add to Notepad
NU-835-BIO14-L 5 x 200 μl (1 mM) 282,50 Add to Basket/Quote Add to Notepad
Structural formula of Biotin-14-dATP (Biotin-14-N6-(6-Amino)hexyl-dATP, Triethylammonium salt)
Structural formula of Biotin-14-dATP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula: C32H54N9O15P3S (free acid)

Molecular Weight: 929.81 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile clear aqueous solution, in 10 mM Tris-HCl pH 7.5 ±0.5

Concentration: 1 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 266 nm; ε 16.2 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I [1] & in-house data

Description:
Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taq polymerase Biotin-11-dATP (#NU-1175-BIOX) is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker.

Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/ 50% dATP

Please note: The optimal final concentration of Biotin-14-dATP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-14-dATP/dATP ratio is recommended.

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Selected References:
[1] Gebeyehu et al. (1987) Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA. Nucleic Acids Res. 15 (21):4513.