Biotin-X-5-Propargylamino-ddUTP
γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2',3'-dideoxyuridine-5'-triphosphate, Triethylammonium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-1619-BIOX-S | 25 μl (1 mM) | 82,80 | Add to Basket/Quote Add to Notepad |
| NU-1619-BIOX-L | 5 x 25 μl (1 mM) | 303,10 | Add to Basket/Quote Add to Notepad |
![Structural formula of Biotin-11-ddUTP (Biotin-X-5-Propargylamino-ddUTP, γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2',3'-dideoxyuridine-5'-triphosphate, Triethylammonium salt)](/images/Structures/NU-1619-BIOX.png)
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C28H43N6O16P3S (free acid)
Molecular Weight: 844.66 g/mol (free acid)
Exact Mass: 844.17 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: filtered solution (30 kDa) in 10 mM Tris-HCl
Color: colorless to slightly yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 290 nm, ε 13.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
SNP-analysis[1]
DNA sequencing[2]
3'end labeling[3]
BIOZ Product Citations:
Selected References:
[1] Tong et al. (2002) Single nucleotide polymorphism detection by combinatorial fluorescence energy transfer tags and biotinylated dideoxynucleotides. Nucleic Acids Res. 30 (5):e19.
[2] Ju (2002) DNA sequencing with solid-phase-capturable dideoxynucleotides and energy transfer primers. Anal Biochem. 309 (1):35.
[3] Wang et al. (2009) Role of sgcR3 in positive regulation of enediyne antibiotic C-1027 production of Streptomyces globisporus C-1027. BMC Microbiol. 9:14.
Edwards et al. (2001) DNA sequencing using biotinylated dideoxynucleotides and mass spectrometry. Nucleic Acids Res. 29 (21):E104.
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