Biotinyl-ε-aminocaproyl-γ-aminobutyryl-5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C32H52N7O18P3S (free acid)
Molecular Weight: 947.78 g/mol (free acid)
Exact Mass: 947.23 g/mol (free acid)
Purity: ≥ 98 % (HPLC)
Form: sterile solution in water
Color: colorless to slightly yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 240 nm, ε 10.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA/cDNA by
- PCR with Taq polymerase [1,2] & in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data
- Primer Extension with Klenow exo- [2,5]
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [6,7]
- Reverse Transcription with MMLV Reverse Transcriptase [8,9]
Incorporation into RNA by
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) 
Biotin-16-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of uridine.
Recommended Biotin-16-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-16-dUTP/ 50% dTTP
Please note: The optimal final concentration of Biotin-16-dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-16-dUTP/dTTP ratio is recommended.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Anderson et al. (2005) Incorporation of reporter-labeled nucleotides by DNA polymerases. Biotechniques 38:257.
 Cross et al. (1990) The structure of a subterminal repeated sequence present on many human chromosomes.Nucleic Acids Res. 18:6649.
 Langer et al. (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc. Natl. Sci. USA 78:6633.
 Brigati et al. (1983) Detection of viral genomes in cultured cells and para nembedded tissue sections using biotin-labeled hybridization probes. Virology 12632.
 Perry et al. (2007) Diet and the evolution of human amylase gene copy number variation. Nat Genet 39:1256.
 Gorczyca et al. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl tranferase and nick translation assays. Caner Res 63:1954.
 Bassell et al. (1994) Single mRNAs visualized by ultrastructural in situ hybridization are principally localized at actin lament intersections in fibroblasts. J Cell Biol 126:863.
 Yih et al. (2002) Changes in gene expression profiles of human fibroblasts in response to sodium arsenite treatment. Carcinogenesis 23:867.
 Luo et al. (2003) Designing, testing, and validating a focused stem cell microarray for characterization of neutral stem cells and progenitor cells. Stem Cells 21:575.
 Rosemeyer et al. (1995) Nonradioactive 3'-End Labeling of RNA molecules of different length by Terminal Deoxynucleotidyltransferase. Analytical Biochemistry 224:446.