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Biotin-11-dATP

γ-[N-(Biotin-6-amino-hexanoyl)]-7-propargylamino-2'-deoxy-7-deaza-adenosine-5'-triphosphate, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-1175-BIOX-S 10 μl (1 mM) 99,24 Add to Basket/Quote Add to Notepad
NU-1175-BIOX-L 5 x 10 μl (1 mM) 383,90 Add to Basket/Quote Add to Notepad
Structural formula of Biotin-11-dATP (γ-[N-(Biotin-6-amino-hexanoyl)]-7-propargylamino-2'-deoxy-7-deaza-adenosine-5'-triphosphate, Triethylammonium salt)
Structural formula of Biotin-11-dATP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C30H45N8O15P3S (free acid)

Molecular Weight: 882.71 g/mol (free acid)

Exact Mass: 882.19 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile clear aqueous solution in 10 mM Tris-HCl

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 280 nm, ε 12.7 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- PCR with Taq polymerase [1] & in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [2]
- Primer Extension with Klenow exo- [3,4]
- Reverse Transcription with MMLV Reverse Transcriptase [5]

Description:
Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.

Recommended Biotin-16-dATP/dATP ratio for PCR: 50% Biotin-16-dATP/ 50% dATP

Please note: The optimal final concentration of Biotin-11-dATP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Biotin-11-dATP/dATP ratio is recommended.

Selected References:
[1] Zammatteo et al. (2005) Unambiguous identification of the expressed MAGE-A genes on a DNA microarray. Clin. Chem. 51 (12):2420.
[2] Dauwerse et al. (1999) Two-colour FISH detection of the inv (16) in interphase nuclei of patients with acute myeloid leukaemia. Br. J. Haematol. 106 (1):111.
[3] Vorwerk et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N. Biotechnol. 25 (2):142.
[4] Beier et al. (2008) Microfluidic primer extension assay. Methods Mol Biol 822:143.
[5] Vankoningsloo et al. (2008) Gene expression silencing with 'specific' small interfering RNA goes beyond specificity - a study of key parameters to take into account in the onset of small interfering RNA off-target effects. FEBS J. 275 (11):2738.