Biotin-14-N6-(6-Aminohexyl)-dATP, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C32H54N9O15P3S (free acid)
Molecular Weight: 929.81 g/mol (free acid)
Exact Mass: 929.27 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile solution in 10 mM Tris-HCl
Color: colorless to slightly yellow
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I  & in-house data
- Primer Extension with Klenow fragment 
Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taq polymerase Biotin-11-dATP (#NU-1175-BIOX) is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker.
Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/ 50% dATP
Please note: The optimal final concentration of Biotin-14-dATP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-14-dATP/dATP ratio is recommended.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Gebeyehu et al. (1987) Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA. Nucleic Acids Res. 15 (21):4513.
 Nagano et al. (2015) Single-cell Hi-C for genome-wide detection of chromatin interactions that occur simultaneously in a single cell. Nature Protocols 10 (12):1987.
Mumbach et al. (2016) HiChIP: Efficient and sensitive analysis of protein-directed genome architecture Nature Protocols 13 (11):919.