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Biotin-14-dCTP

Bio-14-dCTP, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-956-BIO14-S 200 μl (1 mM) 92,25 Add to Basket/Quote Add to Notepad
NU-956-BIO14-L 5 x 200 μl (1 mM) 282,50 Add to Basket/Quote Add to Notepad
Structural formula of Biotin-14-dCTP (Bio-14-dCTP, Triethylammonium salt)
Structural formula of Biotin-14-dCTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C31H54N7O16P3S (free acid)

Molecular Weight: 905.78 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: sterile clear aqueous solution in 10 mM Tris-HCl pH 7.5

Concentration: 1.0 mM - 1.1 mM

Spectroscopic Properties: λmax 272 nm; ε 13.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA/cDNA by
- Nick Translation with DNAse I/ DNA Polymerase I [1] & in-house data
- Primer Extension with Klenow fragment [2]

Description:
Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taq polymerase Biotin-11-dCTP (#NU-809-BIOX) or Biotin-16-dCTP (#NU-809-BIO16) are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively.

Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/ 50% dCTP

Please note: The optimal final concentration of Biotin-14-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-14-dCTP/dCTP ratio is recommended.

Selected References:
[1] Gebeyehu et al. (1987) Novel biotinylated nucleotide-analogs for labeling and colorimetric detection of DNA. Nucleic Acids Res. 15 (21):4513.
[2] Lieberman-Aiden et al. (2009) Comprehensive mapping of long range interactions reveals folding principles of the human genome. Science 326 (5959):289.