2'-O-Methyladenosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H18N5O13P3 (free acid)
Molecular Weight: 521.21 g/mol (free acid)
Exact Mass: 521.01 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.1 L mmol-1 cm-1 (Tris-HCl pH 7.0)
Inhibition of RNA polymerase
Sequence specific incorporation by RNAse P
Nucleotide specifity of CCA-adding enzyme
 Aastroem et al. (2004) Acidity of secondary hydroxyls in ATP and adenosine analogues and the question of 2,3-hydrogen bond in ribonucleosides. J. Am. Chem. Soc. 126 (45):14710.
 Aivazashvill et al. (1977) ATP analogs in the RNA-polymerase reaction. Molecularnaya Biologiya (Moscow) 11 (4):854.
 Conrad et al. (1995) Enzymatic synthesis of 2-modified nucleic acids: identification of important phosphate and ribose moieties in RNase P substrates. Nucleic Acids Res. 23 (11):1845.
 Vaish et al. (2003) A novel, modification-dependent ATP-binding aptamer selected from an RNA library incorporating a cationic functionality. Biochemistry 42 (29):8842.
Fa et al. (2004) Expanding the substrate repertoire of a DNA polymerase by directed evolution. J. Am. Chem. Soc. 126 (6):1748.
Cho et al. (2003) Use of nucleotide analogs by class I and class II CCA-adding enzymes (tRNA nucleotidyltransferase): deciphering the basis for nucleotide selection. RNA 9 (8):970.