5-Bromo-UTP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₉H₁₄N₂O₁₅P₃Br (free acid)

Molecular Weight: 563.03 g/mol (free acid)

Exact Mass: 561.88 g/mol (free acid)

CAS#: 161848-60-8

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 278 nm, ε 9.7 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Agonist for P2Y₂, P2Y₄ and P2Y₆ receptors^[1,2,3]

Selected References:
[1] Wilson et al. (1998) Nucleotide-evoked calcium signals and anion secretion in equine cultured epithelia that express apical P2Y (2) receptors and pyrimidine nucleotide receptors. Brit. J. Pharmacol. 124 (4):832.
[2] Communi et al. (1996) Pharmacological characterization of the human P-2Y4 receptor. Eur. J. Pharmacol. 317 (2-3):383.
[3] El-Tayeb et al. (2006) Synthesis and structure-activity relationships of uracil nucleotide derivatives and analogues as agonists at human P2Y2, P2Y4, and P2Y6 receptors. J. Med. Chem. 49 (24):7076.
Gilles et al. (2007) Regulatory Mechanisms Differ in UMP Kinase from Gram-negative and Gram-positive Bacteria. J. Biol. Chem. 282 (10):7242.
Dunoyer et al. (2001) Peanut clump virus RNA-1-Encoded p15 regulates viral RNA accumulation but is not abundant at viral RNA replication sites. J. Virology 75 (4):1941.
Cmarko et al. (2000) Ultrastructural analysis of nucleolar transcription in cells microinjected with 5-bromo-UTP. Histochem. Cell Biol. 113 (3):181.
Cmarko et al. (1999) Ultrastructural analysis of transcription and splicing in the cell nucleus after bromo-UTP microinjection. Mol. Biol. Cell 10 (1):211.
Keene et al. (1999) Initially transcribed sequences strongly affect the extent of abortive initiation by RNA polymerase II. J. Biol. Chem. 274 (17):11526.
Wilson et al. (1999) Pyrimidine nucleotide-evoked inhibition of cyclic amp accumulation in equine epithelial cells. Exp. Physiol. 84 (4):639.
Mote et al. (1998) Recognition of a human arrest site is conserved between RNA polymerase II and prokaryotic RNA polymerases. J. Biol. Chem. 273 (27):16843.
Schul et al. (1998) Coiled bodies and U2 snRNA genes adjacent to coiled bodies are enriched in factors required for snRNA transcription. Mol. Biol. Cell 9 (5):1025.
Tanner et al. (1988) Binding interactions between yeast tRNA ligase and a precursor transfer ribonucleic acid containing two photoreactive uridine analogues. Biochemistry 27 (24):8852.