UDP-GalNAz

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₇H₂₆N₆O₁₇P₂ (free acid)

Molecular Weight: 648.37 g/mol (free acid)

Exact Mass: 648.08 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solid

Color: colorless to white

Solubility: water, aqueous buffer pH 7-7.5

Spectroscopic Properties: λ_max 262 nm, ε 10.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Transfer of GalNAz moiety to GalNAc residues of proteins and monoclonal antibodies with ß1,4-galactosyltranferase mutant Y289L-Gal-T1 ^[1-2].
The azide-functionalized target can subsequently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce

• a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or
• a fluorophore (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging

Related products:

• UDP-6-azide-glucose, #CLK-076
• Ac4GalNAz, #CLK-1086

BIOZ Product Citations:

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Selected References:
[1] Hang et al. (2004) Probing glycosyltransferase activities with the Staudinger ligation. J. Am. Chem. Soc. 126(1):6.
[2] Qasba et al. (2008) Site-specific linking of biomolecules via glycan residues using glycosyltransferases. Biotechnol. Prog. 24(3):520.