For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C15H23N5O16P2
Molecular Weight: 591.32 g/mol
Purity: ≥ 98 % (HPLC)
Form: white to off-white solid
Solubility: water, aqueous buffer such as PBS
Glycosylation of 5-hydroxymethylcytosine (5-hmC) with T4 ß-Glycosyltransferase (T4 ß-GT)[1-3]
The resulting azide-functionalized, glycosylated 5-hmC moiety can subsequently be detected via Cu(I)-free click chemistry that offers the choice
 Song et al. (2011) Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nature Biotech 29(1):68.
 Li et al. (2012) Selective Capture of 5-hydroxymethylcytosine from Genomic DNA. J. Vis. Exp. 68:e4441.
 Song et al. (2016) Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation. PNAS 113(16):4339.