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Nourseothricin - Powder

NTC or clonNAT

powder (non-sterile)

Cat. No. Amount EUR / g Price (EUR) Buy / Note
AB-102L 1 g176,00 176,00 Add to Basket/Quote Add to Notepad
AB-102XL 5 g169,00 845,00 Add to Basket/Quote Add to Notepad
AB-102-25G 25 g147,60 3.690,00 Add to Basket/Quote Add to Notepad
AB-102-100G 100 g142,00 14.200,00 Add to Basket/Quote Add to Notepad

For research use only! Not intended for human or animal diagnostic or therapeutic uses.

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C

Shelf Life: 36 months

Molecular Formula: C19H34O8N8 * H2SO4 (Streptothricin F)

Molecular Weight: 600 g/mol (Streptothricin F)

CAS#: 96736-11-7

Form: non-sterile beige powder

Description:
Streptothricin antibiotic Nourseothricin (NTC) for a broad spectrum of bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, microalgae, plants and many more. Selection of recombinant strains is based on inactivation of NTC by mono-acetylation of ß-amino group of the ß-lysine by Nouresothricin N-acetyltransferase the product of the sat1 or nat1 genes.

Selection:
For selection of recombinant Leishmania strains Nourseothricin (NTC) is added to growth medium to a final concentration of 100 μg/ml.

For selection of other species please refer to the product page.

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.

Selected References:
[1] Goldstein et al. (1999) Three New Dominant Drug Resistance Cassettes for Gene Disruption in Saccharomyces cerevisiae. Yeast 15: 1541

[2] Kojic et al. (2000) Shuttle vectors for genetic manipulations in Ustilago maydis. Can. J. Microbiology 46: 333

[3] Werner et al. (2001) Aminoglycoside-Streptothricin Resistance Gene Cluster aadE–sat4–aphA-3 Disseminated among multiresistant Isolates of Enterococcus faecium. Antimicrob. Agents Chemotherapy 45: 3267

[4] Hoff et al. (2009) Homologous recombination in the antibiotic producer Penicillium chrysogenum: strain ΔPcku70 shows up-regulation of genes from the HOG pathway. Appl. Microbiol. Biotechnol. 85:1081

[5] Kochupurakkal & Iglehart (2013) Nourseothricin N-Acetyl Transferase: A Positive Selection Marker for Mammalian Cells. PLoS One 8: e68509

[6] Ramos et al. (2013) Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana. Molecular Plant Pathology 14: 44

[7] Schubert et al. (2013) Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus. J. Microbiol. Meth. 95: 251

[8] Buhmann et al. (2014) A Tyrosine-Rich Cell Surface Protein in the Diatom Amphora coffeaeformis Identified through Transcriptome Analysis and Genetic Transformation. PLOS one 9: e110369

[9] Kraeva et al. (2015) Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by Sequencing, Transcriptome Profiling and Co-infection with Leishmania donovani. PLOS Pathogens 11: e1005127

[10] Paschke et al. (2018) Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. PLoS One 13: e0196809