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Azide-containing Fluorescent Dyes

(Picolyl-) Azides of fluorescent dyes can be used for the fluorescent labeling of terminal Alkyne and strained Alknye (e.g DBCO)-labeled molecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

Standard Dyes such as Cy3, Tamra or Texas Red have been thoroughly selected to cover the whole UV-Vis spectrum. Novel Alternative Dyes are available as well.

 

Table 1: Available (Picolyl-) Azide-containing Fluorescent Dyes

Emission
color
DyeAzide
blue3-Azido-7-hydroxycoumarin3-Azido-7-hydroxycoumarin
greenBDP-FL
(also known as BODIPY® FL)
Azide - BDP-FL
5-Fluorescein (5-FAM)5-FAM-Azide
6-Fluorescein (6-FAM)6-FAM-Azide
5/6-Fluorescein (5/6-FAM)Picolyl-Azide/6-FAM
AF488
(also known as Alexa Fluor®488)
AF488-Azide
AF488-Picolyl-Azide
5/6-Carboxyrhodamine 1105/6-Carboxyrhodamine 110-PEG3-Azide
yellow-
green
5-SIMA5-SIMA-Azide
yellow5-TAMRA5-TAMRA-Azide
5/6-TAMRA5/6-TAMRA-PEG3-Azide
Picolyl-Azide-5/6-TAMRA
Cy3Cy3-Azide
Sulfo-Cy3Sulfo-Cy3-Azide
Picolyl-Azide-Sulfo-Cy3
AF546
(also known as Alexa Fluor®546)
AF546-Azide
AF546-Picolyl-Azide
AF555
(structural analog to Alexa Fluor®555)
AF555-Azide
AF555-Picolyl-Azide
orange5/6-Texas Red5/6-Texas Red-PEG3-Azide
AF594
(also known as Alexa Fluor®594)
AF594-Azide
AF594-Picolyl-Azide
redCy5Cy5-Azide
Sulfo-Cy5Sulfo-Cy5-Azide
Picolyl-Azide-Sulfo-Cy5
AF647
(also known as Alexa Fluor®647)
AF647-Azide
AF647-Picolyl-Azide
Cy5.5Cy5.5-Azide
Picolyl-Azide-Cy5.5
near-IRCy7Cy7-Azide
Picolyl-Azide-Cy7

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