Mant-dGTP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₈H₂₃N₆O₁₄P₃ (free acid)

Molecular Weight: 640.33 g/mol (free acid)

Exact Mass: 640.05 g/mol (free acid)

CAS#: 124615-99-2

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 252/355 nm, ε 22.6/5.7 L mmol^-1 cm^-1 (Tris-HCl pH 7.5), λ_exc 355 nm, λ_em 448 nm

Applications:
Resonance energy transfer studies (FRET)^[1]
Enzyme kinetic studies^[2]

Specific Ligands:

Bacillus anthraxis toxin edema factor^[1]

EF-Tu^[3]

Selected References:
[1] Suryanarayana et al. (2009) Distinct interactions of 2'- and 3'-O- (N-methyl)anthraniloyl-isomers of ATP and GTP with the adenylyl cyclase toxin of Bacillus anthracis, edema factor. Biochemical Pharmacology 78 (3):224.
[2] Kettlun et al. (2005) Potato tuber isoapyrases:Substrate specifity, affinity labelling, and proteolytic susceptibility. Phytochemistry 66:975.
[3] Rodnina et al. (1995) Codon-dependent conformational change of elongation-factor tu preceding GTP hydrolysis on the ribosome. EMBO J. 14 (11):2613.
Hazlett et al. (1993) Solution dynamics of p21 (Ras)proteins bound with fluorescent nucleotides - a time-resolved fluorescence study. Biochemistry-US 32 (49):13575.