2'/3'-O-(N-Methyl-anthraniloyl)-guanosine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H23N6O15P3 (free acid)
Molecular Weight: 656.33 g/mol (free acid)
Exact Mass: 656.04 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 252/355 nm, ε 22.6/5.7 L mmol-1 cm-1 (Tris-HCl pH 7.5), λexc 355 nm, λem 448 nm
Inhibition of AC-isoform and GTPs
Activity measurement: GC
Specifity measurements with isoforms of ACs
FRET: AC, edema factor
Inhibition of edema factor (anthrax)
BIOZ Product Citations:
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 Gille et al. (2003) Mant-substituted guanine nucleotides: A novel class of potent adenylyl cyclase inhibitors. Life Sciences 74:271.
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Pinto et al. (2011) Structure-activity relationships for the interactions of 2'- and 3'- (O)- (N-methyl)anthraniloyl-substituted purine and pyrimidine nucleotides with mammalian adenylyl cyclases. Biochem. Pharmacol. 82 (4):358.
Spangler et al. (2011) Interaction of the diguanylate cyclase YdeH of Escherichia coli with 2', (3')-substituted purine and pyrimidine nucleotides. J. Pharmacol. Exp. Ther. 336 (1):234.
Wang et al. (2011) Charge isomers of myelin basic protein: structure and interactions with membranes, nucleotide analogues, and calmodulin. PLoS One. 6 (5):e19915.
Goettle et al. (2010) Cytidylyl and uridylyl cyclase activity of bacillus anthracis edema factor and Bordetella pertussis CyaA. Biochemistry. 49 (26):5494.
Taha et al. (2009) Molecular Analysis of the Interaction of Anthrax Adenylyl Cyclase Toxin, Edema Factor, with 2' (3')-O- (N- (methyl)anthraniloyl)-Substituted Purine and Pyrimidine Nucleotides. Molecular Pharmacology. 75 (3):693.
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Brownbridge et al. (1993) Interaction of GTPase activating proteins (GAPs) with p21ras measured by a novel fluorescence anisotropy method. Essential role of Arg-903 of GAP in activation of GTP hydrolysis on p21ras. J. Biol. Chem. 268:10914.
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