NBD-GPP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark

Shelf Life: 12 months

Molecular Formula: C₁₆H₂₂N₄O₁₀P₂

Molecular Weight: 492.31 g/mol

Purity: ≥ 90 % (HPLC)

Form: liquid (Supplied as 1 mM solution in 25 mM (NH₄)₂CO₃)

Concentration: 1 mM

Spectroscopic Properties: λ_exc 480 nm, λ_em 530 nm, ε 11.0 L mmol^-1 cm^-1 (pH 7.5)

Description:
NBD-GPP is a fluorescent analog of geranyl pyrophosphate (GPP). It serves as lipid donor for farnesyltransferase (FTase) - one of the three mammalian protein prenyltransferases. The attachment of the NBD-geranyl group to protein substrates allows for efficient fluorescence based FTase activity assays and inhibitor screening.

BIOZ Product Citations:

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Selected References:
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227.
Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704.
Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.