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NBD-GPP

3,7-dimethyl-8-(7-nitro-benzo[1,2,5]oxadiazol-4-ylamino),
-octa-2,6-diene-1- pyrophosphate

Fluorescent lipid donor for protein farnesyltransferase (FTase)

Cat. No. Amount Price (EUR) Buy / Note
LI-014 20 μl 188,90 Add to Basket/Quote Add to Notepad
Structural formula of NBD-GPP (3,7-dimethyl-8-(7-nitro-benzo[1,2,5]oxadiazol-4-ylamino),-octa-2,6-diene-1- pyrophosphate)
Structural formula of NBD-GPP

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark

Shelf Life: 12 months

Molecular Formula: C16H22N4O10P2

Molecular Weight: 492.31 g/mol

Purity: ≥ 95 % (HPLC)

Form: liquid (Supplied as 1 mM solution in 25 mM (NH4)2CO3)

Concentration: 1 mM

Spectroscopic Properties: λexc 480 nm, λem 530 nm, ε 11.0 L mmol-1 cm-1 (pH 7.5)

Description:
NBD-GPP is a fluorescent analog of geranyl pyrophosphate (GPP). It serves as lipid donor for farnesyltransferase (FTase) - one of the three mammalian protein prenyltransferases. The attachment of the NBD-geranyl group to protein substrates allows for efficient fluorescence based FTase activity assays and inhibitor screening.

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.

Selected References:
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227.
Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704.
Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.