Fluorescent lipid donor for protein farnesyltransferase (FTase)
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles, store dark
Shelf Life: 12 months
Molecular Formula: C16H22N4O10P2
Molecular Weight: 492.31 g/mol
Purity: ≥ 95 % (HPLC)
Form: liquid (Supplied as 1 mM solution in 25 mM (NH4)2CO3)
Concentration: 1 mM
Spectroscopic Properties: λexc 480 nm, λem 530 nm, ε 11.0 L mmol-1 cm-1 (pH 7.5)
NBD-GPP is a fluorescent analog of geranyl pyrophosphate (GPP). It serves as lipid donor for farnesyltransferase (FTase) - one of the three mammalian protein prenyltransferases. The attachment of the NBD-geranyl group to protein substrates allows for efficient fluorescence based FTase activity assays and inhibitor screening.
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Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227.
Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704.
Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.