Protein farnesyltransferase, α- and β-subunit
rat, recombinant, E. coli
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For general laboratory use.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: α: 44 kDa, β: 49 kDa
Accession number: Q04631 / Q02293
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl, 1 mM TCEP and 10 μM ZnSO4)
Activity: 1 pmol of FTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
FTase catalyzes the transfer of the farnesyl group from farnesyl diphosphate to proteins containing a C-terminal CaaX motif, where 'C' is a conserved cysteine that is the site of farnesyl modification, 'a' is usually an aliphatic amino acid, and 'X' is methionine, serine, glutamine, or alanine.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
Lackner et al. (2005) Chemical genetics indentifies Rab geranylgeranyl transferase as an apoptotic target of farnesyl transferase inhibitors. Cancer Cell. 7:325.
Zimmerman et al. (1998) High-level expression of rat farnesyl:proteintransferase in Escherichia coli as a translationally coupled heterodimer. Protein Express. Purif. 14:395.
Hooff et al. (2008) Isoprenoid quantitation in human brain tissue: a validated HPLCfluorescence detection method for endogenous farnesyl- (FPP) and geranylgeranylpyrophosphate (GGPP). Analytical and Bioanalytical Chemistry. 392 (4):673-680.
Watanabe et al. (2008) Inhibitors of Protein Geranylgeranyltransferase I and Rab Geranylgeranyltransferase Identified from a Library of Allenoate-derived Compounds. J. Biol. Chem. 283 (15):9571-9579.
Eckert et al. (2009) Regulation of the brain isoprenoids farnesyl- and geranylgeranylpyrophosphate is altered in male Alzheimer patients. Neurobiology of Disease 35 (2):251-257.