Innovative lipid donors have been developed for sensitive prenylation assays. NBD-FPP and NBD-GPP are a well-established fluorescent analogs of the natural lipid donors.
B-GPP is the first universal lipid donor for all prenylatable proteins, providing versatile prenylation assays with femtomolar detection sensitivity.
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Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704
Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822