H-Ras

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Description:
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and K-Ras4B). Suitable as a substrate for farnesyltransferase. Protein preparation is 98% GDP- and 2% GTP-loaded, measured by HPLC.

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 21.3 kDa (189 amino acids)

Accession number: NP_005334

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl, 3 mM DTT, 2 mM MgCl₂ and 0.01 mM GDP)

Activity: GTPγS binding: >750 mmol/mol.

Selected References:
Sasazuki et al. (2005) Transformation by Oncogenic RAS Sensitizes Human Colon Cells to TRAIL-induced Apoptosis by Up-regulating Death Receptor 4 and Death Receptor 5 through a MEK-dependent Pathway. J Biol. Chem. 280:22856.
Marshall (1995) Ras target proteins in eukaryotic cells. FASEB J. 9:1311.
Reiss et al. (1990) Inhibition of purified p21ras farnesyl-protein transferase by Cys-aaX tetrapeptides. Cell 62:81.
Chaussade et al. (2009) Functional differences between two classes of oncogenic mutation in the PIK3CA gene. Biochemical and Biophysical Research Communications 381 (4):577-581.