γ-[N-(Biotin-6-amino-hexanoyl)]-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C28H45N6O17P3S (free acid)
Molecular Weight: 862.67 g/mol (free acid)
Exact Mass: 862.18 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: sterile clear aqueous solution in 10 mM Tris-HCl
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 240/289 nm, ε 10.7/7.1 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA/cDNA by
- PCR with Taq polymerase [1,2] & in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data
- Primer Extension with Klenow 3'-5' exo- 
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)
- Reverse Transcription with MMLV Reverse Transcriptase 
Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine.
Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/ 50% dTTP
Please note: The optimal final concentration of Biotin-11-dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dUTP/dTTP ratio is recommended.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Day et al. (1990) Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction. Biochem J 267:119.
 Ried et al. (1992) Simultaneous visualization of severe different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc. Natl. Acad. Sci. USA 89:1388.
 Langer et al. (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633.
 Brigati et al. (1983) Detection of viral genomes in cultured cells and para nembedded tissue sections using biotin-labled hybridization probes. Virology 126:32.
 Day et al. (2008) Microfluidic-based enzymatic on-chip labeling of miRNAs. N Biotechnol 25:142.
 Flickinger et al. (1992) Differential incorporation of biotinylated nucleotides by terminal deoxynucleotidyl transferase. Nucleic Acids Res 20:2382.
 Anderson et al. (2005) Incorporation of reporter-labeled nucleotides by DNA polymerases. Biotechniques 38:257.
Moritz et al. (2014) Simple methods for the 3' biotinylation of RNA. RNA. 20 (3):421.