3'-dATP
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O12P3 (free acid)
Molecular Weight: 491.18 g/mol (free acid)
Exact Mass: 491.00 g/mol (free acid)
CAS#: 73-04-1
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
3'-end labeling of RNA with recombinant yeast poly(A) polymerase[1]
3'-end labeling of oligonucleotide primers[2]
Selected References:
[1] Lingner et al. (1993) 3'-end labeling of RNA with recombinant yeast poly (A) polymerase Nucleic Acids Research 21 (12):2917.
[2] Austermann et al. (1992) Inhibition of human immunodeficiency virus type 1 reverse transcriptase by 3'-blocked oligonucleotide primers. Biochem. Pharmacol. 43 (12):2581.
Ozsolak et al. (2009) Direct RNA sequencing. Nature 461 (7265):814.
Shuman (1995) Vaccinia virus DNA ligase: specificity, fidelity, and inhibition. Biochemistry 34 (49):16138.
Labruyere et al. (1990) Characterization of ATP and calmodulin-binding properties of a truncated form of Bacillus anthracis adenylate cyclase. Biochemistry 29 (20):4922.
Freist et al. (1983) Isoleucyl-tRNA synthetase from Baker's yeast. Catalytic mechanism, 2',3'-specificity and fidelity in aminoacylation of tRNAIle with isoleucine and valine investigated with initial-rate kinetics using analogs of tRNA, ATP and amino acids. Eur J Biochem. 131 (1):65.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-1123S | 50 μl (10 mM) | 157,60 € |
| NU-1123L | 5 x 50 μl (10 mM) | 461,30 € |
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Scientific citations for Jena Bioscience nu-1123
21 scientific citations and 1 citation images found by Bioz.
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Click-code-seq reveals strand biases of DNA oxidation and depurination in human genome.
Nature Chemical Biology, 2025-10-31 · PMID:41174235
.. H2O and Therminator IX (NEB (custom synthesis), 10 U per μl) to the sample.. In case of quantifying AP sites, 3 μl of H2O was replaced by
3 μl ofprop-dATP (Jena Biosciences , custom synthesis,2.5 mM ).. After 10 min of incubation at 60 °C, gDNA samples were purified using Pronex .. -
Molecular mechanism of co-transcriptional H3K36 methylation by SETD2.
Nature Communications, 2025-10-30 · PMID:41162378
.. (400nM) was added and the reaction was incubated on ice for a further 10min.. DSIF (800 nM), SPT6 (800 nM), PAF1c (800nM), RTF1 (800 nM), P-TEFb (400 nM), AKT3 (400 nM), IWS1 (2.4 μM), SETD2 (1.6 μM),
3’dATP (0.5mM) (Jena Bioscience ), FACT (800 nM), and water was added.. Compensation buffer was then added to bring the reaction final conditions to those listed .. -
Structural insights into human Pol III transcription initiation in action.
Nature, 2025-06-04 · PMID:40468065
Abstract: RNA polymerase III (Pol III) transcribes highly demanded RNAs grouped into three types of classical promoters, including type 1 (5S rRNA), type 2 (tRNA) and type 3 (short non-coding RNAs, such as U6, 7SK and RNase H1) promoters 1-7 . While structures of the Pol III preinitiation complex (PIC) 8-11 and elongation complex (EC) 12-16 have been determined, the mechanism underlying the transition from initiation to elongation remains unclear. Here we reconstituted seven human Pol III transcribing complexes (TC4, TC5, TC6, TC8, TC10, TC12 and TC13) halted on U6 promoters with nascent RNAs of 4-13 nucleotides
