3'-Deoxyadenosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H16N5O12P3 (free acid)
Molecular Weight: 491.18 g/mol (free acid)
Exact Mass: 491.00 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
3'-end labeling of RNA with recombinant yeast poly(A) polymerase
3'-end labeling of oligonucleotide primers
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Lingner et al. (1993) 3'-end labeling of RNA with recombinant yeast poly (A) polymerase Nucleic Acids Research 21 (12):2917.
 Austermann et al. (1992) Inhibition of human immunodeficiency virus type 1 reverse transcriptase by 3'-blocked oligonucleotide primers. Biochem. Pharmacol. 43 (12):2581.
Ozsolak et al. (2009) Direct RNA sequencing. Nature 461 (7265):814.
Shuman (1995) Vaccinia virus DNA ligase: specificity, fidelity, and inhibition. Biochemistry 34 (49):16138.
Labruyere et al. (1990) Characterization of ATP and calmodulin-binding properties of a truncated form of Bacillus anthracis adenylate cyclase. Biochemistry 29 (20):4922.
Freist et al. (1983) Isoleucyl-tRNA synthetase from Baker's yeast. Catalytic mechanism, 2',3'-specificity and fidelity in aminoacylation of tRNAIle with isoleucine and valine investigated with initial-rate kinetics using analogs of tRNA, ATP and amino acids. Eur J Biochem. 131 (1):65.