2'/3'-O-(2-Aminoethyl-carbamoyl)-adenosine-5'-triphosphate, labeled with Cy3, Triethylammonium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-808-CY3 | 160 μl (1 mM) | 1.150,70 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C44H58N9O21P3S2 (free acid)
Molecular Weight: 1206.03 g/mol (free acid)
Exact Mass: 1205.24 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: pink
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Fluorescence polarization[1]
Stopped-flow studies[1]
Kinetics of nucleotide exchange[2-4]
Fluorescence correlation spectroscopy[5]
Crossbridging by flash photolysis[6]
Specific Ligands:
Myosin/actomyosin-ATPase[1]
Myofibrils[2]
BIOZ Product Citations:
Selected References:
[1] Oiwa et al. (2003) The 2-O- and 3-O-Cy3-EDA-ATP (ADP) complexes with myosin subfragment-1 are spectroscopically distinct. Bioph. J. 84:634.
[2] Chaen et al. (1998) Measurement of ATP turnover during shortening and lengthening of rabbit psoas myofibrils using fluorescent ATP analog. Adv. Experim. Medicine and Biology 453:569.
[3] Shirakawa et al. (2000) Measurements of nucleotide exchange rate constants in single rabbit soleus myofibrils during shortening and lengthening using a fluorescent ATP analog. Biophysival J. 78:918.
[4] Oiwa et al. (1998) Direct observation of a central bare zone in a native thick filament isolated from the anterior byssus retractor muscle of Mytilus edulis, using fluorescent ATP analog. J. Biochem. 123:614.
[5] Kuhlmann et al. (1998) ATPase kinetics of Dictostelium discoideum myosin II motor domain. J. Muscle Res.and Cell Motility 19:491.
[6] Chaen et al. (1998) Measurement of ATP turnover during shortening and lengthening of rabbit psoas myofibrils using fluorescent ATP analog. Adv. Experim. Medicine and Biology 453:569.
Nishizaka et al. (2011) Simultaneous observation of chemomechanical coupling of a molecular motor. Methods Mol Biol. 778:259.
Yanagida et al. (2011) Single-molecule measurements using microneedles. Methods Mol Biol. 778:143.
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