2'/3'-O-(N-Methyl-anthraniloyl)-adenosine-5'-triphosphate, Triethylammonium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-202S | 150 μl (10 mM) | 135,60 | Add to Basket/Quote Add to Notepad |
| NU-202L | 5 x 150 μl (10 mM) | 396,80 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H23N6O14P3 (free acid)
Molecular Weight: 640.33 g/mol (free acid)
Exact Mass: 640.05 g/mol (free acid)
CAS#: 151481-86-6
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 255/355 nm, ε 23.3/5.8 L mmol-1 cm-1 (Tris-HCl pH 7.5), λexc 355 nm, λem 448 nm
Applications:
Conformational dynamic: DnaB/C-protein[1], Csk[2]
Inhibition of AC-isoforms[3]
Fluorescence stop-flow kinetics: PKA[4]
FRET: AC[5], myosin V[6]
Specific Ligands:
DnaC-protein[7]
Factor Rho[8]
BIOZ Product Citations:
Selected References:
[1] Roychowdhury et al. (2009) Interactions of Escherichia coli DnaB-DnaC protein complex with nucleotide cofactors. 1. Allosteric conformational transitions of the complex. Biochemistry 48:6712.
[2] Shaffer et al. (2001) Nucleotide release and associated conformational changes regulate function in the COOH-terminal Src kinase, Csk. Biochemistry 40:11149.
[3] Gille et al. (2004) Differential inhibition of adenylyl cyclase isoforms and soluble guanylyl cyclase by purine and pyrimidine nucleotides. J. Biol. Chem. 279:19955.
[4] Ni et al. (2000) Insights into nucleotide binding in protein kinase A using fluorescent adenosine derivatives. Protein Science 9:1818.
[5] Goettle et al. (2007) Molecular analysis of the interaction of Bordetella pertussis adenylyl cyclase with fluorescent nucleotides. Molecular Pharmacology 72 (3):526.
[6] Sun et al. (2006) Dynamics of the upper 50-kDa domain of myosin V examined with fluorescence resonance energy transfer. J. Biol. Chem. 281:5711.
[7] Galletto et al. (2005) The nucleotide-binding site ot the Escherichia coli DnaC protein: Molecular topography of DnaC protein-nucleotide cofactor complex. Cell Biochem. And Biophys. 43:331.
[8] Jeong et al. (2004) Nucleotide binding induces conformational changes in Escherichia coli transcription termination factor Rho. J. Biol. Chem. 279:1837.
Seifert et al. (2012) Inhibitors of membranous adenylyl cyclases.Trends Pharmacol. Sci. 33 (2):64.
Wang et al. (2011) Charge isomers of myelin basic protein: structure and interactions with membranes, nucleotide analogues, and calmodulin. PLoS One. 6 (5):e19915.
Pinto et al. (2011) Structure-activity relationships for the interactions of 2'- and 3'- (O)- (N-methyl)anthraniloyl-substituted purine and pyrimidine nucleotides with mammalian adenylyl cyclases. Biochem. Pharmacol. 82 (4):358.
Hübner et al. (2011) Effect of MANT-nucleotides on L-type calcium currents in murine cardiomyocytes. J. Naunyn Schmiedebergs Arch. Pharmacol. 383 (4):573.
Spangler et al. (2011) Interaction of the diguanylate cyclase YdeH of Escherichia coli with 2', (3')-substituted purine and pyrimidine nucleotides. J. Pharmacol. Exp. Ther. 336 (1):234.
Erdorf et al. (2011) Pharmacological characterization of adenylyl cyclase isoforms in rabbit kidney membranes. J. Naunyn Schmiedebergs Arch. Pharmacol. 383 (4):357.
Goettle et al. (2010) Cytidylyl and uridylyl cyclase activity of bacillus anthracis edema factor and Bordetella pertussis CyaA. Biochemistry 49 (26):5494.
Chen et al. (2009) Structural Basis of Mechanochemical Coupling in a Hexameric Molecular Motor. J. Biol. Chem. 283 (6):3607.
Kainov et al. (2008) ADP but Not Pi Dissociation Contributes to Rate Limitation for Escherichia coli Rho. J. Biol. Chem. 284 (49):33773.
Kambach et al. (2007) Human OLA1 Defines an ATPase subfamily in the Obg Family of GTP-binding proteins. J. Biol. Chem. 282 (27):19928.
Scott et al. (2006) Chain-Terminating Dinucleoside Tetraphosphates are substrates for DNA Polymerization by Human Immunodefiency Virus Type 1 Reverse Transcriptase with Increased Activity against Thymidine Analogue-Resistant Mutants. AAC 50 (11):3607.
Lísal et al. (2005) Cooperative Mechanism of RNA Packaging Motor. J. Biol. Chem. 280 (24):23157.
Tung-Chung Mou et al. (2005) Structural Basis for the Inhibition of Mammalian Membrane Adenylyl Cyclase by 2' (3')-O- (N-Methylanthraniloyl)-guanosine 5'-Triphosphate. J. Biol. Chem. 280 (8):7253.
Fujita et al. (1999) Fluorescence changes of a label attached near the myosin active site on nucleotide binding in rat skeletal muscle fibres. J. Physiol. 515:869.
Thoenges et al. (1999) Tight binding of bulky fluorescent derivatives of adenosine to the low affinity E2ATP site leads to inhibition of Na+/K+-ATPase. Analysis of structural requirements of fluorescent ATP derivatives with a Koshland-Nemethy-Filmer model of two interacting ATP sites. J. Biol. Chem. 274:1971.
Cheng et al. (1998) Interaction of mant-adenosine nucleotides and magnesium with kinesin. Biochemistry 37:5288.
Mocz et al. (1998) Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue 2' (3')-O- (-N-Methylanthraniloyl)-adenosine 5'-triphosphate. Biochemistry 37:9862.
Jameson et al. (1997) Fluorescent nucleotide analogs: synthesis and applications. Methods Enzymol. 278:363.
Hiratsuka (1983) New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes. Biochim. Biophys. Acta 742:496.
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