Mant-ATP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₈H₂₃N₆O₁₄P₃ (free acid)

Molecular Weight: 640.33 g/mol (free acid)

Exact Mass: 640.05 g/mol (free acid)

CAS#: 151481-86-6

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 255/355 nm, ε 23.3/5.8 L mmol^-1 cm^-1 (Tris-HCl pH 7.5), λ_exc 355 nm, λ_em 448 nm

Applications:
Conformational dynamic: DnaB/C-protein^[1], Csk^[2]
Inhibition of AC-isoforms^[3]
Fluorescence stop-flow kinetics: PKA^[4]
FRET: AC^[5], myosin V^[6]

Specific Ligands:

DnaC-protein^[7]

Factor Rho^[8]

Selected References:
[1] Roychowdhury et al. (2009) Interactions of Escherichia coli DnaB-DnaC protein complex with nucleotide cofactors. 1. Allosteric conformational transitions of the complex. Biochemistry 48:6712.
[2] Shaffer et al. (2001) Nucleotide release and associated conformational changes regulate function in the COOH-terminal Src kinase, Csk. Biochemistry 40:11149.
[3] Gille et al. (2004) Differential inhibition of adenylyl cyclase isoforms and soluble guanylyl cyclase by purine and pyrimidine nucleotides. J. Biol. Chem. 279:19955.
[4] Ni et al. (2000) Insights into nucleotide binding in protein kinase A using fluorescent adenosine derivatives. Protein Science 9:1818.
[5] Goettle et al. (2007) Molecular analysis of the interaction of Bordetella pertussis adenylyl cyclase with fluorescent nucleotides. Molecular Pharmacology 72 (3):526.
[6] Sun et al. (2006) Dynamics of the upper 50-kDa domain of myosin V examined with fluorescence resonance energy transfer. J. Biol. Chem. 281:5711.
[7] Galletto et al. (2005) The nucleotide-binding site ot the Escherichia coli DnaC protein: Molecular topography of DnaC protein-nucleotide cofactor complex. Cell Biochem. And Biophys. 43:331.
[8] Jeong et al. (2004) Nucleotide binding induces conformational changes in Escherichia coli transcription termination factor Rho. J. Biol. Chem. 279:1837.
Seifert et al. (2012) Inhibitors of membranous adenylyl cyclases.Trends Pharmacol. Sci. 33 (2):64.
Wang et al. (2011) Charge isomers of myelin basic protein: structure and interactions with membranes, nucleotide analogues, and calmodulin. PLoS One. 6 (5):e19915.
Pinto et al. (2011) Structure-activity relationships for the interactions of 2'- and 3'- (O)- (N-methyl)anthraniloyl-substituted purine and pyrimidine nucleotides with mammalian adenylyl cyclases. Biochem. Pharmacol. 82 (4):358.
Hübner et al. (2011) Effect of MANT-nucleotides on L-type calcium currents in murine cardiomyocytes. J. Naunyn Schmiedebergs Arch. Pharmacol. 383 (4):573.
Spangler et al. (2011) Interaction of the diguanylate cyclase YdeH of Escherichia coli with 2', (3')-substituted purine and pyrimidine nucleotides. J. Pharmacol. Exp. Ther. 336 (1):234.
Erdorf et al. (2011) Pharmacological characterization of adenylyl cyclase isoforms in rabbit kidney membranes. J. Naunyn Schmiedebergs Arch. Pharmacol. 383 (4):357.
Goettle et al. (2010) Cytidylyl and uridylyl cyclase activity of bacillus anthracis edema factor and Bordetella pertussis CyaA. Biochemistry 49 (26):5494.
Chen et al. (2009) Structural Basis of Mechanochemical Coupling in a Hexameric Molecular Motor. J. Biol. Chem. 283 (6):3607.
Kainov et al. (2008) ADP but Not Pi Dissociation Contributes to Rate Limitation for Escherichia coli Rho. J. Biol. Chem. 284 (49):33773.
Kambach et al. (2007) Human OLA1 Defines an ATPase subfamily in the Obg Family of GTP-binding proteins. J. Biol. Chem. 282 (27):19928.
Scott et al. (2006) Chain-Terminating Dinucleoside Tetraphosphates are substrates for DNA Polymerization by Human Immunodefiency Virus Type 1 Reverse Transcriptase with Increased Activity against Thymidine Analogue-Resistant Mutants. AAC 50 (11):3607.
Lísal et al. (2005) Cooperative Mechanism of RNA Packaging Motor. J. Biol. Chem. 280 (24):23157.
Tung-Chung Mou et al. (2005) Structural Basis for the Inhibition of Mammalian Membrane Adenylyl Cyclase by 2' (3')-O- (N-Methylanthraniloyl)-guanosine 5'-Triphosphate. J. Biol. Chem. 280 (8):7253.
Fujita et al. (1999) Fluorescence changes of a label attached near the myosin active site on nucleotide binding in rat skeletal muscle fibres. J. Physiol. 515:869.
Thoenges et al. (1999) Tight binding of bulky fluorescent derivatives of adenosine to the low affinity E2ATP site leads to inhibition of Na+/K+-ATPase. Analysis of structural requirements of fluorescent ATP derivatives with a Koshland-Nemethy-Filmer model of two interacting ATP sites. J. Biol. Chem. 274:1971.
Cheng et al. (1998) Interaction of mant-adenosine nucleotides and magnesium with kinesin. Biochemistry 37:5288.
Mocz et al. (1998) Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue 2' (3')-O- (-N-Methylanthraniloyl)-adenosine 5'-triphosphate. Biochemistry 37:9862.
Jameson et al. (1997) Fluorescent nucleotide analogs: synthesis and applications. Methods Enzymol. 278:363.
Hiratsuka (1983) New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes. Biochim. Biophys. Acta 742:496.