Adenosine-5'-(γ-thio)-triphosphate, Tetralithium salt
For research use only!
Shipping: shipped on dry ice
Storage Conditions: store at -20 °C
Shelf Life: 6 months after date of delivery
Molecular Formula: C10H16N5O12P3S (free acid)
Molecular Weight: 523.24 g/mol (free acid)
Exact Mass: 522.97 g/mol (free acid)
Purity: ≥ 90 % (HPLC), contains < 10 % ADP
Color: white to off-white
Spectroscopic Properties: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Modulation of intracellular signalling[1,2]
Signalling of purinergic receptors
Regulation of cation channels
Modulation of cytokine secretion
Substrate for kinases
5' end labeling with T4 Polynucleotide Kinase (T4 PNK) of DNA and RNA[9,10]
Agonistic ligand, mainly for nucleoside receptor A1
Nucleosidephosphates stabilized against hydrolytic degradation can directly bind to nucleoside receptors.
Agonist for P2Y2 and 12 receptors[1,15] and for P2X(2) purinoreceptor[3,12,13,14]
Please note: For reasons of stability, please make sure that the pH value of a solution of this product never drops below 7.0. This can be achieved by dissolving the nucleotide in a buffer of your choice (50 - 100 mM, pH 7 - 10). Dissolve and adjust concentration photometrically.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Buzzi et al. (2009) Extracellular ATP activates MAP kinase cascades through a P2Y purinergic receptor in the human intestinal Caco-2 cell line. Biochim. Biophys. Acta 1790:1651.
 Wu et al. (2008) Extracellular ATP-induced NO production and ist dependence on membrane Ca2+ flux in Salvia miltiorrhiza hairy roots. J. Experim. Botany 59:4007.
 Hansen et al. (2008) Purinergic receptors and calcium signalling in human pancreatic duct cell lines. Cell. Phys. Biochem. 22:157.
 Ashrafpour et al. (2008) ATP regulation of a large conductance voltage-gated cation channel in rough endoplasmatic reticulum of rat hepatocytes. Arch. Biochem. Biophys. 471:50.
 Nalos et al. (2008) Host tissue damage signal ATP impairs IL-12 and IFNgamma secretion in LPS stimulated whole human blood. Intensive care medicine 34:1891.
 Carlson et al. (2010) Use of a semisynthetic epitope to probe histidine kinase activity and regulation. Anal. Biochem. 397:139.
 Choi et al. (2009) Modulation of firing activity by ATP in dopamine neurons of the substantia nigra pars compacta. Neuroscience 160:587.
 Wang et al. (2009) Structural insights on the mycobacterium tuberculosis protosomal ATPase Mpa. Structure 17:1377.
 Grant et al. (2007) A facile method for attaching nitroxide spin labels at the 5' terminus of nucleic acids. Nucleic Acids Research 35 (10):e77.
 Akabayov et al. (2002) Immobilization of RNA: Application to Single Molecule Spectroscopy. Molecular, Cellular and Tissue Engineering, 2002. Proceedings of the IEEE-EMBS Special Topic Conference:71.
 Gorodeski (2002) Regulation of transcervical permeability by two distinct P2 purinergic receptor mechanisms. Am. J. Physiol. Cell Physiol. 282 (1):C75.
 Bo et al. (1994) Comparative studies on affinities of ATP derivatives for P2X-purinoreceptors in rat urinary bladder. Br. J. Pharmacol. 112 (4):1151.
He et al. (2002) Purinergic P2X (2) receptor desensitization depends on coupling between ectodomain and C-terminal domain. Molec. Pharmac. 62 (5):1187.
Evans et al. (1995) Pharmacological characterizationof heterogously expressed ATP-gated cation channels (P2X purinoreceptors). Mol. Pharmacol. 48:178.
 Isfort et al. (2011) Real-time imaging reveals that P2Y2 and P2Y12 receptor agonists are not chemoattractants and macrophage chemotaxis to complement C5a is phosphatidylinositol 3-kinase (PI3K)- and p38 mitogen-activated protein kinase (MAPK)-independent. J. Biol. Chem. 288 (52):44776.
Schmohl et al. (2012) Functional analysis of Rho GTPase activation and inhibition in a bead-based miniaturized format. Methods Mol. Biol. 827:271.
Fischmann et al. (2009) Crystal Structures of MEK1 Binary and Ternary Complexes with Nucleotides and Inhibitors. Nucleic Acids Research 48 (12):2661.
Del Toro Duany et al. (2008) The reverse gyrase helicase-like domain is a nucleotide-dependent switch that is attenuated by the topoisomerase domain. Nucleic Acids Research 36 (18):5882.
Wong et al. (2006) Endogenous activation of adenosine A1 receptors, but not P2X receptors, during high frequency synaptic transmission at the calyx of Held. J. Neurophysiology 95 (6):3336.
Chang et al. (2005) Nitric Oxide-dependent Allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Matamoros et al. (2005) Suppression of Multidrug-resistant HIV-1 Reverse Transcriptase Primer Unblocking Activity by α-Phosphate-modified Thymidine Analogues. J. Mol. Biol. 349 (3):451.
Masino et al. (2002) Modulation of hippocampal glutamatergic transmission by ATP is dependent on adenosine A1 receptors. J. Pharmacol. and Experiment. Therapeutics 303 (1):356.