d4TTP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₀H₁₅N₂O₁₃P₃ (free acid)

Molecular Weight: 464.15 g/mol (free acid)

Exact Mass: 463.98 g/mol (free acid)

CAS#: 611-60-9

Purity: ≥ 95 % (HPLC)

Form: solution in water

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 266 nm, ε 10.1 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Selected References:
Coulier et al. (2008) Simultaneous determination of endogenous deoxynucleotides and phosphorylated nucleoside reverse transcriptase inhibitors in peripheral blood mononuclear cells using ion-pair liquid chromatography coupled to mass spectrometry. Proteomics Clin. Appl. 2:1557.
Ray et al. (2002) Insights into the molecular mechanism of inhibition and drug resistance for HIV-1 RT with carbovir triphosphate. Biochemistry 41:5150.
Hoggard et al. (2000) Correlation between intracellular pharmacological activation of nucleoside analogues and HIV suppression in vitro. Antivir. Chem. Chemother. 11:353.
Vaccaro et al. (2000) Mechanism of inhibition of the human immunodeficiency virus type 1 reverse transcriptase by d4TTP: an equivalent incorporation efficiency relative to the natural substrate dTTP. Antimicrob. Agents. Chemother. 44:217.
Ueno et al. (1997) Comparative enzymatic study of HIV-1 reverse transcriptase resistant to 2',3'-dideoxynucleotide analogs using the single-nucleotide incorporation assay. Biochemistry 36:1092.
Im et al. (1993) Identification of the amino acid in the human immunodeficiency virus type 1 reverse transcriptase involved in the pyrophosphate binding of antiviral nucleoside triphosphate analogs and phosphonoformate. Implications for multiple drug resistance. Biochem. Pharmacol. 46:2307.
Huang et al. (1992) Selective action of 2',3'-didehydro-2',3'-dideoxythymidine triphosphate on human immunodeficiency virus reverse transcriptase and human DNA polymerases. J. Biol. Chem. 267:2817.