for inducible expression
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Upon arrival the glycerol stocks must be stored at -80 °C or inoculated into LEXSY BHI.
Shelf Life: 12 months
The Leishmania tarentolae strain T7-TR is used as host strain for inducible LEXSY expression vectors.
3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host T7-TR (recombinant Leishmania tarentolae strain expressing bacteriophage T7 RNA polymerase and TET repressor).
These stocks can be stored at -80 °C for at least 1 year. For reactivation see below.
Recombinant Leishmania tarentolae strain constitutively expressing bacteriophage T7 RNA polymerase and TET repressor.
1, Non-pathogenic for mammalians
Reactivation of LEXSY host:
Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (Cat.-No. ML-411) containing the antibiotics Nourseothricin (Cat.-No. AB-101) and LEXSY Hygro (Cat.-No. AB-104). Incubate at 26 °C and dilute as required.
Preparation of LEXSY BHI growth medium:
Dissolve 37 g/l LEXSY BHI powder (Cat.-No. ML-412) in deionized water and autoclave for 15 min at 121 °C. Add Hemin and PenStrep. Store at 4 °C and use within two weeks.
Immediately before use, add the antibiotics Nourseothricin (Cat.-No. AB-101) and LEXSY Hygro. (Cat.-No. AB-104) for maintenance of the T7 polymerase and TET repressor genes.
Preparation of glycerol stocks:
Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80 % glycerol, mix, incubate 10 min at RT, 1 h on ice and over night at -20 °C. Transfer to -80 °C. Strains can be stored this way for several years.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
 Gruba et al. (2019) Development of Chemical Tools to Monitor Human Kallikrein 13 (KLK13) Activity. Int J Mol Sci. 20: 1557