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LEXSY BHI - Powder Media Kit

brain-heart infusion-based

recommended for transfection and strain maintenance

Cat. No. Amount Price (EUR) Buy / Note
ML-412S for 1 L 49,62 Add to Basket/Quote Add to Notepad
ML-412L for 5 L 190,04 Add to Basket/Quote Add to Notepad
ML-412XL for 10 L 369,51 Add to Basket/Quote Add to Notepad
ML-412XXL for 50 L 1.478,05 Add to Basket/Quote Add to Notepad

For research use only! Not intended for human or animal diagnostic or therapeutic uses.

Shipping: Shipped on blue ice

Storage Conditions: Store components as indicated on individual labels

Shelf Life: 12 months

Applications:
For the growth of LEXSY hosts P10 (Cat.-No. LT-101) and T7-TR (Cat.-No. LT-110).

Description:
Three-component Kit for preparation of complete Leishmania cultivation medium.

Content:
LEXSY BHI, powder

Hemin stock solution, 500x (Cat.-No. ML-108):
0,25% solution of Hemin in 30% Triethanolamine, sterile

Pen-Strep stock solution, 200x (Cat.-No. ML-105):
10.000 U/ml of penicillin G sodium salt and 10.000 μg/ml of streptomycin sulfate in 0.85 % saline, sterile

Cat. No. Content Amount
ML-412S 37 g powder
2 ml Hemin
5 ml Pen-Strep
for 1 l medium
ML-412L 185 g powder
10 ml Hemin
25 ml Pen-Strep
for 5 l medium
ML-412XL 370 g powder
20 ml Hemin
50 ml Pen-Strep
for 10 l medium
ML-412XXL 1850 g powder
100 ml Hemin
250 ml Pen-Strep
for 50 l medium

Storage conditions:
LEXSY BHI, powder:
Store at room temperature
Stable for 12 months

Hemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 month

Pen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month


Preparation of complete Leishmania cultivation medium:

Dissolve 37 g/l LEXSY BHI powder in deionized water and autoclave for 15 min at 121 °C. Control temperature and time with a reference with the same volume, since overexposition leads to de-composition of ingredients and a dark brown color of the medium, which adversely affects growth of LEXSY strains. Proper autoclaved medium is of amber color. Store medium at room temperature before addition of Hemin and Pen-Strep (stable for 12 month). As an alternative, the medium can also be sterile filtered.

Add to 1 l LEXSY BHI:
5.0 ml Pen-Strep solution
2.0 ml Hemin stock solution to a final concentration of 5 μg/ml

Final pH = 7.6 ± 0.2

Store in the dark at 4 °C.

Completed medium is useable for up to 2 weeks. If the completed medium is to be used after this period, appropriate amounts of Hemin and Pen-Strep have to be re-added.

Selected References:
[1] Zauner et al. (2018) Structural Analyses of Arabidopsis thaliana Legumain γ Reveal Differential Recognition and Processing of Proteolysis and Ligation Substrates. J Biol Chem 293: 8934