NTC or clonNAT
sterile ready-to-go stock solution, 100 mg/ml
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
Shipping: Shipped on blue ice
Storage Conditions: Store at -20 °C
Shelf Life: 12 months
Molecular Formula: C19H34N8O8 ∙ H2SO4 (Streptothricin F)
Molecular Weight: 600.6 g/mol (Streptothricin F)
Form: Beige liquid
Concentration: 100 mg/ml of sterile filtrated Nourseothricin in water
Nourseothricin is a mixturte of Streptothricins C, D, E and F and can be used as selection antibiotic for a broad spectrum of pro- and eukaryotic organisms (i.e. Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, microalgae, plants and many more).
Selection of recombinant strains is based on inactivation of Nourseothricin by mono-acetylation of the ß-amino group of the ß-lysine residue by Nourseothricin N-acetyltransferase, the product of the sat1 or nat1 genes.
For selection of recombinant Leishmania strains Nourseothricin is added to the growth medium to a final concentration of 100 μg/ml.
For selection of other species please refer to the product page.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Goldstein et al. (1999) Three New Dominant Drug Resistance Cassettes for Gene Disruption in Saccharomyces cerevisiae. Yeast 15: 1541
 Kojic et al. (2000) Shuttle vectors for genetic manipulations in Ustilago maydis. Can. J. Microbiology 46: 333
 Werner et al. (2001) Aminoglycoside-Streptothricin Resistance Gene Cluster aadE–sat4–aphA-3 Disseminated among multiresistant Isolates of Enterococcus faecium. Antimicrob. Agents Chemotherapy 45: 3267
 Hoff et al. (2009) Homologous recombination in the antibiotic producer Penicillium chrysogenum: strain ΔPcku70 shows up-regulation of genes from the HOG pathway. Appl. Microbiol. Biotechnol. 85:1081
 Kochupurakkal & Iglehart (2013) Nourseothricin N-Acetyl Transferase: A Positive Selection Marker for Mammalian Cells. PLoS One 8: e68509
 Ramos et al. (2013) Functional genomics tools to decipher the pathogenicity mechanisms of the necrotrophic fungus Plectosphaerella cucumerina in Arabidopsis thaliana. Molecular Plant Pathology 14: 44
 Schubert et al. (2013) Agrobacterium-mediated transformation of the white-rot fungus Physisporinus vitreus. J. Microbiol. Meth. 95: 251
 Buhmann et al. (2014) A Tyrosine-Rich Cell Surface Protein in the Diatom Amphora coffeaeformis Identified through Transcriptome Analysis and Genetic Transformation. PLOS one 9: e110369
 Kraeva et al. (2015) Leptomonas seymouri: Adaptations to the Dixenous Life Cycle Analyzed by Sequencing, Transcriptome Profiling and Co-infection with Leishmania donovani. PLOS Pathogens 11: e1005127
 Paschke et al. (2018) Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum. PLoS One 13: e0196809