Spin-column based DNA cleanup from PCR samples
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at ambient temperature
Shelf Life: 12 months
PCR Purification Kit is designed for the work-up of PCR reactions (removal of primer dimers, primers, nucleotides, proteins, salt, agarose, ethidium bromide, and other impurities). The preparation is based on a silica-membrane technology for binding DNA in high-salt and elution in low-salt buffer. The kit provides a simple and efficient way to purify linear or circular DNA in the size range from 100 bp to 10 kb and is optimized for working with DNA amounts of up to 20 μg. It requires no organic extractions or precipitation and guarantees high and stable recovery rates.
Washing Buffer (before use, add 96-99 % Ethanol as indicated on the bottle)
2 ml Collection Tubes
To be provided by you:
96-99 % Ethanol
Isopropanol (for high yield sample preparation)
1.5 ml microtubes
The DNA purification follows a simple binding, washing and eluting procedure. Before start, add 96-99 % Ethanol to the Washing Buffer as indicated on the bottle.
|Binding Buffer||30 ml||150 ml|
|Activation Buffer||6 ml||30 ml|
|Washing Buffer||add 64 ml Ethanol |
(final volume 80 ml)
|add 160 ml Ethanol to each bottle (final volume 200 ml each)|
|Elution Buffer||5 ml||25 ml|
The additional use of Isopropanol is recommended for fragments smaller than 200 bp or larger than 5 kbp. The optional secondary washing step minimizes the salt content of the purification product but may significantly reduce the yield of DNA fragments <200 bp.
1a Standard Sample Preparation:
For DNA fragment sizes in the range of 200 bp to 5 kbp:
1b High Yield Sample Preparation:
For DNA fragment sizes smaller than 200 bp or larger than 5 kbp:
2 Column Activation:
3 Column Loading:
4 Column Washing:
Optional Secondary Washing: Recommended only for DNA >200 bp, if highly purified DNA (for DNA sequencing, transfection etc.) is required.
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