γ-(2-Azidoethyl)-adenosine-5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H19N8O13P3 (free acid)
Molecular Weight: 576.25 g/mol (free acid)
Exact Mass: 576.03 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
in vitro phosphorylation of recombinant proteins
Lee et al. reported a non-radioactive version of in vitro phosphorylation were γ-[2-Azidoethyl]-ATP (compound 8) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2.
The phosphorylated, azide-modified protein substrate can subsequently be labeled with Alkynes of biotin or fluorescent dyes via Cu(I)- catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu(I)-free Click-Chemistry.
Presolski et al. and Hong et al. provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Lee et al. (2009) Synthesis and reactivity of novel γ-phosphate modified ATP analogues. Bioorg Med Chem Lett. 19:3804.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.