5-Ethynyl-dUTP (5-EdUTP)
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H15N2O14P3 (free acid)
Molecular Weight: 492.16 g/mol (free acid)
Exact Mass: 491.97 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1]
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice
- to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks
- to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging
- to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins
Related products:
- Copper (II)-Sulphate (CuSO4), #CLK-MI004
- Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA), #CLK-1010
- Sodium Ascorbate (Na-Ascorbate), #CLK-MI005
Selected References:
[1] Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.
[2] Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.
[3] Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
[4] Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.
| Cat. No. | Amount | Price |
|---|---|---|
| CLK-T07-S | 10 μl (100 mM) | 117,50 € |
| CLK-T07-L | 5 x 10 μl (100 mM) | 352,50 € |
| CLK-T07-XL | 100 μl (100 mM) | 612,80 € |
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Scientific citations for Jena Bioscience clk-t07
8 scientific citations and 0 citation images found by Bioz.
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Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells
Scientific Reports, 2023-04-08 · PMID:37031254
5-ethynyl-dUTP (EdUTP) was used instead of dTTP in PCR and the resulting PCR yielded products with proper fluorophores, while biotin was introduced by „Click-it “ azide-ethynyl chemical reaction using kits fromJena Biosciences (Jena, Germany).. Fluorophore-containing mtFISH probes were prepared by PCR using Alexa Fluor 647-dUTP (A647-dUTP) from the .. -
Possible frequent multiple mitochondrial DNA copies in a single nucleoid
Europe PMC, 2022-09-22 · DOI:10.21203/rs.3.rs-2063370
Analyses of STED-visualized nucleoid diameters were performed using the CellPro ler software (https://cellpro ler.org/; RRID: SCR_007358). mtDNA uorescence in situ hybridization (mtFISH) protocol 5-ethynyl-dUTP (EdUTP) was used instead of dTTP in PCR and the resulting PCR yielded products with proper uorophores, while biotin was introduced by „Click-it“
azide-ethynyl chemical reaction using kits fromJena Biosciences (Jena, Germany). -
Glycosylated oligonucleotides that mimic the HIV-1 epitope of neutralizing monoclonal antibody 2G12
, 2020-09-22 · DOI:
Examples of modified nucleosides that can be introduced during this extension step include, without limitation, N6-(6-azido)hexyl-dATP (Jena Bioscience), C8-alkyne-dCTP (Jena Bioscience),
5-ethynyl-dUTP (Jena Bioscience ), C8-alkyne-dUTP (Jena Bioscience), 5-azido-C3-UTP (Jena Bioscience), 5-ethynyl-UTP (Jena Bioscience), N6-propargyl-ATP (Jena Bioscience), 2-ethynyl-ATP (Jena Bioscience), and 8-azido-ATP.
