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CLICK-functionalized Nucleotides for DNA Labeling


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

 

Table 1: Enzymatic incorporation of CLICK-functionalized nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase (CLK-T09, NU-1705) or family B polymerases Pwo, Deep Vent exo- and KOD XL (CLK-T05/-T06/-T07); NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with family B polymerases Pwo, Deep Vent exo- and KOD XL; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT:: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)


DNA LabelingcDNA
Labeling
NucleotideCat. No.PCR NTPrimer Extension3’ End LabelingRT
dUTP
5-DBCO-PEG4-dUTPCLK-0481n/an/an/an/a
5-Azidomethyl-dUTPCLK-084n/an/an/an/an/a
Azide-PEG4-Aminoallyl-dUTPNU-17051n/an/a2n/a
5-Ethynyl-dUTPCLK-T071,2n/a2n/an/a
C8-Alkyne-dUTPCLK-T051,2n/a2n/an/a
5-TCO-PEG4-dUTPCLK-0351n/an/an/an/a
5-Vinyl-dUTPCLK-0681n/an/an/an/a
dCTP
C8-Alkyne-dCTPCLK-T061,2n/a22n/a
5-DBCO-PEG4-dCTPCLK-0601n/an/an/an/a
5-Azido-PEG4-dCTPCLK-0701n/an/an/an/a
dATP
N6-Azidohexyl-dATPCLK-NU-0021n/an/an/an/a
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable