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Amine-modified Nucleotides for DNA Labeling


Nucleotide Selector for DNA/RNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA/RNA Labeling.

 

Table 1: Enzymatic incorporation of Amine-modified nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase; NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with Klenow 3'-5'exo-; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)


DNA LabelingcDNA
Labeling
NucleotideCat. No.PCR NTPrimer Extension3’ End LabelingRT
dUTP
5-Aminoallyl-dUTPNU-8031,22222
dCTP
5-Propargylamino-dCTPNU-8091n/an/an/an/a
dATP
N6-6-Aminohexyl-dATP
N6 position
NU-835-2n/an/an/a
7-Deaza-7-Propargylamino-dATP
7-Deaza position
NU-16112n/an/an/an/a
1: in-house tested
2: external reference (see datasheet)
n/a: no data available
- : not applicable