Digoxigenin presents a good alternative to the biotin-avidin system, mainly because of its very low non-specific binding.[1,2] Detection of hybridized digoxigenin-labeled probes is achieved either by high-affinity anti-digoxigenin antibodies conjugated to either a reporter enzyme (e.g. alkaline phosphatase (AP) or horseradish peroxidase (HRP)) or a fluorescent dye or by a secondary antibody that is similarly labeled.
Find Digoxigenin-labled nucleotides and Labeling Kits suitable for enzymatic DNA Labeling or enzymatic RNA Labeling.
[1] McQuaid et al. (1995) A comparison of digoxigenin and biotin labelled DNA and RNA probes for in situ hybridization. Biotech Histochem.70: 147.
[2] Chevalier et al. (1997) Biotin and digoxigenin as labels for light and electron microscopy in situ hybridization probes: where do we stand? J. Histochem. Cytochem.45: 481.
