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5-Fluoro-UTP

(5F-UTP)

5-Fluoro-uridine-5'-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-1115S 10 μl (100 mM) 123,24 Add to Basket/Quote Add to Notepad
NU-1115L 5 x 10 μl (100 mM) 360,95 Add to Basket/Quote Add to Notepad
Structural formula of 5-Fluoro-UTP ((5F-UTP), 5-Fluoro-uridine-5'-triphosphate, Sodium salt)
Structural formula of 5-Fluoro-UTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C9H14N2O15P3F (free acid)

Molecular Weight: 502.13 g/mol (free acid)

Exact Mass: 501.96 g/mol (free acid)

CAS#: 3828-96-4

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 267 nm, ε 10.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Inhibition of viral application[1]
Mutagenic activity[1]
Conformational studies (NMR-NOE) of HIV-2 TAR-RNA[2]
Use as NMR-label (F)[2]
X-ray of viral RNA polymerase[3]

Selected References:
[1] Agudo et al. (2008) Molecular Characterization of a Dual Inhibitory and Mutagenic Activity of 5-Fluorouridine Triphosphate on Viral RNA Synthesis. Implications for Lethal Mutagenesis. J. Mol. Biol. 382:652.
[2] Hennig et al. (2007) Synthesis of 5-fluoropyrimidine nucleotides as sensitive NMR probes of RNA structure. J. Am. Chem. Soc. 129:14911.
[3] Ferrer-Orta et al. (2007) Sequential structures provide insights into the fidelity of RNA replication. PNAS USA 104:9463.
Gilles Labesse et al. (2011) Structural and functional characterization of the Mycobacterium tuberculosis uridine monophosphate kinase: insights into the allosteric regulation. Nucleic Acids Res. 39 (8):3458.
Gilles et al. (2007) Regulatory Mechanisms Differ in UMP Kinases from Gram-negative and Gram-positive Bacteria. J. Biol. Chem. 282 (10):7242.
Au et al. (1982) Reversed-phase ion-pair high-performance liquid-chromatographic assay of 5-fluorouracil, 5'-deoxy-5- fluorouridine, their nucleosides, monophosphate, diphosphate, and triphosphate nucleotides with a mixture of quaternary ammonium-ions. J. Chromatogr. 228:245.
Glazer et al. (1980) The effect of 5-fluorouridine 5'-triphosphate on RNA transcribed in isolated-nuclei invitro. Mol. Pharmacol. 17 (2):279.
Anukarahanonta et al. (1979) Enhancement of 5-fluorouridine action in hepatoma-cells by galactosamine-induced uridine triphosphate deficiency. H-S Z. Physiol. Chem. 360 (3):225.