5-Propargylamino-dCTP - Solution

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₂H₁₉N₄O₁₃P₃ (free acid)

Molecular Weight: 520.22 g/mol (free acid)

Exact Mass: 520.02 g/mol (free acid)

CAS#: 115899-39-3

Purity: ≥ 93 % (HPLC)

Form: solution in water

Color: slightly yellow

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 294 nm, ε 9.3 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Incorporation into DNA by

• PCR with Taq polymerase^{in-house data}

Description:
Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice

• to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks
• to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

BIOZ Product Citations:

Powered by Bioz See more details on Bioz