5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt
|Cat. No.||Amount||Price (EUR)||Buy / Note|
|NU-809S||10 μl (100 mM)||67,40||Add to Basket/Quote Add to Notepad|
|NU-809L||5 x 10 μl (100 mM)||304,40||Add to Basket/Quote Add to Notepad|
|NU-809XL||100 μl (100 mM)||505,50||Add to Basket/Quote Add to Notepad|
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H19N4O13P3 (free acid)
Molecular Weight: 520.22 g/mol (free acid)
Exact Mass: 520.02 g/mol (free acid)
Purity: ≥ 93 % (HPLC)
Form: solution in water
Color: slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA by
Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice
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