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5-Methyl-CTP

m5CTP

5-Methylcytidine-5'-triphosphate, Sodium salt

5-Me-CTP

Cat. No. Amount Price (EUR) Buy / Note
NU-1138S 10 μl (100 mM) 111,70 Add to Basket/Quote Add to Notepad
NU-1138L 5 x 10 μl (100 mM) 327,20 Add to Basket/Quote Add to Notepad
Structural formula of 5-Methyl-CTP (m5CTP, 5-Methylcytidine-5'-triphosphate, Sodium salt)
Structural formula of 5-Methyl-CTP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C10H18N3O14P3 (free acid)

Molecular Weight: 497.18 g/mol (free acid)

Exact Mass: 497.00 g/mol (free acid)

CAS#: 327174-86-7 (acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 277 nm, ε 9.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:
Functional influence of modified bases[1-5]
Incorporation by PCR[3]

Related products: HighYield T7 RNA Synthesis Kit, #RNT-101

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.

Selected References:
[1] Adams et al. (2014) 185 CELLULAR VIABILITY AND EXPRESSION OF GREEN FLUORESCENT PROTEIN IN BOVINE FETAL FIBROBLASTS FOLLOWING TRANSFECTION OF SYNTHETIC mRNA INCLUDING MODIFIED BASES. Reprod. Fertil. Dev. 26:207.
[2] Tchigvintsev et al. (2013) Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides. Chem. Biol. Oxford U.K. 20:1386.
[3] Wong et al. (1997) A novel method for producing partial restriction digestion of DNA fragments by PCR with 5-methyl-CTP. Nucleic Acids Res. 25:4169.
[4] Kormann et al. (2011) Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nature Biotechnology 29:154.
[5] Warren et al. (2010) Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7 (5):618.