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2'/3'-O-(2-Aminoethyl-carbamoyl)-Guanosine-5'-triphosphate, labeled with 5/6-TAMRA, Triethylammonium salt

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NU-820-TAM 160 μl (1 mM) 1.150,70 Add to Basket/Quote Add to Notepad
Structural formula of EDA-GTP-5/6-TAMRA (2'/3'-O-(2-Aminoethyl-carbamoyl)-Guanosine-5'-triphosphate, labeled with 5/6-TAMRA, Triethylammonium salt)
Structural formula of EDA-GTP-5/6-TAMRA
excitation and emission spectrum of 5/6-TAMRA
excitation and emission spectrum of 5/6-TAMRA

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C38H42N9O19P3 (free acid)

Molecular Weight: 1021.72 g/mol (free acid)

Exact Mass: 1021.19 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: pink to red

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 545 nm, λem 575 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Microtubule Gliding Assay [1,2]

BIOZ Product Citations:

Selected References:
[1] Martin (2013) Measuring microtubule persistence length using a microtubule gliding assay. Methods Cell Biol. 115:13.
[2] Martin et al. (2012) Flexural rigidity measurements of biopolymers using gliding assays. J. Vis. Exp. 69.
Fujimura et al. (2017) Dissection of the angle of single fluorophore attached to the nucleotide in corkscrewing microtubules. Biochem. Biophys. Res. Commun. 485 (3):614.
Lang et al. (2017) Actin ADP-ribosylation at Threonine148 by Photorhabdus luminescens toxin TccC3 induces aggregation of intracellular F-actin. Cell. Microbiol. 19 (1): e12636.
Anderson et al. (2011) A fluorescent GTP analog as a specific, high-precision label of microtubules. BioTechniques 51 (1):43.
Ahmadian et al. (2005) Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins. Biol. Chem. 386:1105.