5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with Cy3, Triethylammonium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C43H55N6O20P3S2 (free acid)
Molecular Weight: 1132.97 g/mol (free acid)
Exact Mass: 1132.21 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in 10 mM Tris-HCl
Concentration: 1.0 mM - 1.1 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA/cDNA by
- Primer Extension with Klenow fragment
- PCR with Taq polymerase in-house data
- Nick Translation with DNAse I/ DNA Polymerase I in-house data
5-Propargylamino-dCTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine.
Recommended Propargylamino-dCTP-Cy3/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy3/ 70-50% dCTP
Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.
 Walsh et al. (2017) Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy. Nucleic Acids Res. 45 (21):e175.
Ramsay et al. (2010) CyDNA: Synthesis and Replication of Highly Cy-Dye
Substituted DNA by an Evolved Polymerase. J. Am. Chem. Soc. 132 (14):5096.