Mant-AMP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₈H₂₁N₆O₈P (free acid)

Molecular Weight: 480.37 g/mol (free acid)

Exact Mass: 480.12 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 255/355 nm, ε 23.3/5.8 L mmol^-1 cm^-1 (Tris-HCl pH 7.5), λ_exc 355 nm, λ_em 448 nm

Applications:
Stopped-flow kinetic studies^{[1, 2]}
Analysis of conformational changes^[1]

Specific Ligands:

Pyrophosphatase mtCBS^[1]

Helicase DnaB^[2]

BIOZ Product Citations:

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Selected References:
[1] Jaemsen et al. (2010) Nucleotide- and substrate-induced conformational transitions in the CBS domain-containing pyrophosphatase of Moorella thermoacetica. Biochemistry 49:1005.
[2] Bujalowski et al. (2000) Kinetic mechanism of nucleotide cofactor binding to Escherichia coli replicative helicase DnaB protein. Stopped-flow kinetic studies using fluorescent, ribose-, and base-modified nucleotide analogues. Biochemistry 39:2106.
Wang et al. (2011) Charge isomers of myelin basic protein: structure and interactions with membranes, nucleotide analogues, and calmodulin. PLoS One. 6 (5):e19915.
Lísal et al. (2008) Cooperative Mechanism of RNA Packaging Motor. The Journal of biological chemistry 280 (24):23157.
Hiratsuka et al. (1983) New Ribose-modified Fluorescent Analogs of Adenine and Guanine Nucleotides Available as Substrates for Various Enzxmes. Biochim Biophys Acta 742:496.