2'/3'-(N-Methyl-anthraniloyl)-adenosine-5'-monophosphate, Triethylammonium salt
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| NU-236S | 150 μl (10 mM) | 157,60 | Add to Basket/Quote Add to Notepad |
| NU-236L | 5 x 150 μl (10 mM) | 461,70 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C18H21N6O8P (free acid)
Molecular Weight: 480.37 g/mol (free acid)
Exact Mass: 480.12 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 10 mM - 11 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 255/355 nm, ε 23.3/5.8 L mmol-1 cm-1 (Tris-HCl pH 7.5), λexc 355 nm, λem 448 nm
Applications:
Stopped-flow kinetic studies[1, 2]
Analysis of conformational changes[1]
Specific Ligands:
Pyrophosphatase mtCBS[1]
Helicase DnaB[2]
BIOZ Product Citations:
Selected References:
[1] Jaemsen et al. (2010) Nucleotide- and substrate-induced conformational transitions in the CBS domain-containing pyrophosphatase of Moorella thermoacetica. Biochemistry 49:1005.
[2] Bujalowski et al. (2000) Kinetic mechanism of nucleotide cofactor binding to Escherichia coli replicative helicase DnaB protein. Stopped-flow kinetic studies using fluorescent, ribose-, and base-modified nucleotide analogues. Biochemistry 39:2106.
Wang et al. (2011) Charge isomers of myelin basic protein: structure and interactions with membranes, nucleotide analogues, and calmodulin. PLoS One. 6 (5):e19915.
Lísal et al. (2008) Cooperative Mechanism of RNA Packaging Motor. The Journal of biological chemistry 280 (24):23157.
Hiratsuka et al. (1983) New Ribose-modified Fluorescent Analogs of Adenine and Guanine Nucleotides Available as Substrates for Various Enzxmes. Biochim Biophys Acta 742:496.
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