» Sign in / Register

2'OMe-ATP

2'-O-Methyladenosine-5'-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-1184S 50 μl (100 mM) 91,44 Add to Basket/Quote Add to Notepad
NU-1184L 5 x 50 μl (100 mM) 353,73 Add to Basket/Quote Add to Notepad
Structural formula of 2'OMe-ATP (2'-O-Methyladenosine-5'-triphosphate, Sodium salt)
Structural formula of 2'OMe-ATP

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C11H18N5O13P3 (free acid)

Molecular Weight: 521.21 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 259 nm, ε 15.1 L mmol-1 cm-1 (Tris-HCl pH 7.0)

Applications:
Acidity constants[1]
Inhibition of RNA polymerase[2]
Sequence specific incorporation by RNAse P[3]
Nucleotide specifity of CCA-adding enzyme[4]

Selected References:
[1] Aastroem et al. (2004) Acidity of secondary hydroxyls in ATP and adenosine analogues and the question of 2,3-hydrogen bond in ribonucleosides. J. Am. Chem. Soc. 126 (45):14710.
[2] Aivazashvill et al. (1977) ATP analogs in the RNA-polymerase reaction. Molecularnaya Biologiya (Moscow) 11 (4):854.
[3] Conrad et al. (1995) Enzymatic synthesis of 2-modified nucleic acids: identification of important phosphate and ribose moieties in RNase P substrates. Nucleic Acids Res. 23 (11):1845.
[4] Vaish et al. (2003) A novel, modification-dependent ATP-binding aptamer selected from an RNA library incorporating a cationic functionality. Biochemistry 42 (29):8842.
Fa et al. (2004) Expanding the substrate repertoire of a DNA polymerase by directed evolution. J. Am. Chem. Soc. 126 (6):1748.
Cho et al. (2003) Use of nucleotide analogs by class I and class II CCA-adding enzymes (tRNA nucleotidyltransferase): deciphering the basis for nucleotide selection. RNA 9 (8):970.