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Mant-GTPγS

2'/3'-O-(N-Methyl-anthraniloyl)-guanosine-5'-(γ-thio)-triphosphate, Triethylammonium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-209S 10 μl (10 mM) 126,24 Add to Basket/Quote Add to Notepad
NU-209L 5 x 10 μl (10 mM) 369,76 Add to Basket/Quote Add to Notepad
Structural formula of Mant-GTPγS (2'/3'-O-(N-Methyl-anthraniloyl)-guanosine-5'-(γ-thio)-triphosphate, Triethylammonium salt)
Structural formula of Mant-GTPγS

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 6 months after date of delivery

Molecular Formula: C18H23N6O14P3S (free acid)

Molecular Weight: 672.39 g/mol (free acid)

CAS#: 136749-24-1, 136749-26-3

Purity: ≥ 90 % (HPLC)

Form: colorless to slightly yellow solution in water

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λmax 252/355 nm, ε 22.6/5.7 L mmol-1 cm-1 (Tris-HCl pH 7.5), λexc 355 nm, λem 448 nm

Applications:
Inhibition of AC5[1], ACs[2]

Specific Ligands:

Initiation factor elF5B[3]

Selected References:
[1] Rottlaender et al. (2007) Functional adenylyl cyclase inhibition in murine cardiomyocytes by 2 (3)-O- (N-methylanthraniloyl)-guanosine 5- (gamma-thio) triphosphate. J. Pharmacol. Exper. Ther. 321:608.
[2] Gille et al. (2003) Mant-substituted guanine nucleotides: A novel class of potent adenylyl cyclase inhibitors. Life Sciences 74:271.
[3] Pisareva et al. (2007) Kinetic analysis of the interaction of guanine nucleotides with eukariotic translation initiation factor eIF5B. Biochemistry 46:2622.
Pinto et al. (2011) Structure-activity relationships for the interactions of 2'- and 3'- (O)- (N-methyl)anthraniloyl-substituted purine and pyrimidine nucleotides with mammalian adenylyl cyclases. Molecular Pharmacology 82 (4):358.
Hübner et al. (2011) Effect of MANT-nucleotides on L-type calcium currents in murine cardiomyocytes. J. Naunyn Schmiedebergs Arch. Pharmacol. 383 (4):573.
Erdorf et al. (2011) Pharmacological characterization of adenylyl cyclase isoforms in rabbit kidney membranes. J. Naunyn Schmiedebergs Arch. Pharmacol. 383 (4):357.
Spangler et al. (2011) Interaction of the diguanylate cyclase YdeH of Escherichia coli with 2', (3')-substituted purine and pyrimidine nucleotides. J. Pharmacol. Exp. Ther. 336 (1):234.
Koshiba et al. (2011) Structure-function analysis of the yeast mitochondrial Rho GTPase, Gem1p: implications for mitochondrial inheritance. J. Biol. Chem. 286 (1):354.
Goettle et al. (2009) Characterization of Mouse Heart Adenylyl Cyclase. J. Pharmacol. Exp. Ther. 329 (3):1156.
Hunn et al. (2008) Regulatory interactions between IRG resistance GTPases in the cellular response to Toxoplasma gondii. The EMBO Journal 27:2495.
Villarroya et al. (2008) Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification. Molecular and cellular biology 28 (24):7514.
Herzig et al. (2007) Functional Adenylyl Cyclase Inhibition in Murine Cardiomyocytes by 2' (3')-O- (N-Methylanthraniloyl)-Guanosine 5'-[gamma-Thio]triphosphate. J. Pharmacol. Exp. Ther. 321:608.
Goettle et al. (2007) Molecular analysis of the interaction of Bordetella pertussis adenylyl cyclase with fluorescent nucleotides. Molecular Pharmacology 72 (3):526.
Celda et al. (2007) Structural insights into GTPase domain of Escherichia coli MnmE protein. Proteins 66 (3):726.
Armengod et al. (2005) Effects of Mutagenesis in Switch I Region and Conserved Arginines of Escherichia coli MnmE Protein, A GTPase Involved in tRNA Modification. J. Biol. Chem. 280 (35):30660.
Gille et al. (2003) 2' (3')-O- (N-methylanthraniloyl)-substituted GTP analogs: a novel class of potent competitive adenylyl cyclase inhibitors. J. Biol. Chem. 278:12672.
Remmers et al. (1999) Interdomain interactions regulate GDP release from heterotrimeric G proteins. Biochemistry 38 (42):13795.
Remmers et al. (1998) Detection and quantitation of heterotrimeric G proteins by fluorescence resonance energy transfer. Anal. Biochem. 257 (1):89.