Mant-dGDP

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₁₈H₂₂N₆O₁₁P₂ (free acid)

Molecular Weight: 560.35 g/mol (free acid)

Exact Mass: 560.08 g/mol (free acid)

CAS#: 127383-33-9

Purity: ≥ 95 % (HPLC)

Form: solution in water

Concentration: 10 mM - 11 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_max 252/355 nm, ε 22.6/5.7 L mmol^-1 cm^-1 (Tris-HCl pH 7.5), λ_exc 355 nm, λ_em 448 nm

Applications:
Resonance energy transfer to Trp in protein cdc42hs^[1]
Conformational analysis of Ras-like protein^[2]

Specific Ligands:

Ras-like GTP-binding protein Cdc42Hs^{[1, 2]}

Selected References:
[1] Leonard et al. (1994) Investigation of the GTP-binding GTPase cycle of cdc42hs using fluorescence spectroscopy. Biochemistry 33 (40):12323.
[2] Nomanbhoy et al. (1996) Investigation of the GTP-binding GTPase cycle of Cdc42Hs using extrinsic reporter group fluorescence. Biochemistry 35 (14):4602.
Liu et al. (2008) Ras Is Required for the Cyclic AMP-Dependent Activation of Rap1 via Epac2. Molecular and cellular biology 28 (23):7109.