for constitutive expression
For research use only! Not intended for human or animal diagnostic or therapeutic uses.
Shipping: Shipped on dry ice
Storage Conditions: Store at -80 °C
Avoid freeze/thaw cycles
Upon arrival the glycerol stocks must be stored at -80 °C or inoculated into LEXSY BHI.
Shelf Life: 12 months
The Leishmania tarentolae laboratory strain P10 is used as host strain for the constitutive LEXSY expression vectors.
3 vials with 1.6 ml each of frozen glycerol stocks of LEXSY host P10 (Leishmania tarentolae laboratory strain P10).
These stocks can be stored at -80 °C for at least 1 year. For reactivation see below.
Leishmania tarentolae, laboratory strain P10
1, Non-pathogenic for mammalians
Reactivation of LEXSY host:
Thaw glycerol stock on ice and inoculate the entire content of the vial into 10 ml of LEXSY BHI medium (Cat.-No. ML-411). Incubate at 26 °C and dilute as required.
Preparation of LEXSY BHI growth medium:
Dissolve 37 g/l LEXSY BHI powder (Cat.-No. ML-412) in deionized water and autoclave for 15 min at 121 °C. Add Hemin and PenStrep. Store at 4 °C and use within two weeks.
Preparation of glycerol stocks:
Add 1.2 ml of growing culture (ca. 6x107 cells/ml) to one vial with 0.4 ml 80 % glycerol, mix, incubate 10 min at RT, 1 h on ice and over night at -20 °C. Transfer to -80 °C. Strains can be stored this way for several years.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Zauner et al. (2018) Structural Analyses of Arabidopsis thaliana Legumain γ Reveal Differential Recognition and Processing of Proteolysis and Ligation Substrates. J Biol Chem 293: 8934