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JBScreen PACT++

JBScreen PACT++ is a crystallization screen facilitating systematic pH, anion- and cation testing in the presence of polyethylene glycol (PEG) based on the work of Newman et al. [1].

The 96 unique crystallization conditions combine three mini-screens in one:

1. 24-condition PEG/pH screen
2. 24-condition PEG/cation screen
3. 48-condition PEG/anion screen

This systematic approach aims to alter individual components of the crystallization conditions, i.e. pH, anions and cations, independently from the others in order to obtain more information of the precipitation behaviour of the protein.

When JBScreen PACT++ is used along with JBScreen JCSG++, systematic investigation of the precipitation behaviour of the protein can be combined with a sparse matrix screen in order to enhance the success rate of protein crystallization.

Format

Bulk – 24 or 96 screening solutions in 10 ml aliquots
HTS – 96 screening solutions delivered in a deep-well block, 1.7 ml per well

 

Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes.

BIOZ Product Citations

Please click the arrow on the right to expand the citation list. Click publication title for the full text.


Further Selected Literature Citations of JBScreen PACT++

  • Tam et al. (2022) Unidirectional mannitol synthesis of Acinetobacter baumannii MtlD is facilitated by the helix–loop–helix-mediated dimer formation. PNAS 119(14):e2107994119.
  • Kwon et al. (2021) A Novel, Selective c‑Abl Inhibitor, Compound 5, Prevents Neurodegeneration in Parkinson’s Disease. J. Med. Chem. 64:15091.
  • Giunta et al. (2020) Tuning the Properties of Natural Promiscuous Enzymes by Engineering Their Nano-environment. ACS Nano 14:17652.
  • Sugiura et al. (2020) Bacterial inducible expression of plant cell wall‑binding protein YesO through conflict between Glycine max and saprophytic Bacillus subtilis. Scientific Reports 10:18691.
  • Reisky et al. (2019) A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan. Nat. Chem. Biol. 15:803.
  • Demmer et al. (2017) The semiquinone swing in the bifurcating electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile. Nat. Commun. DOI: 10.1038/s41467-017-01746-3.

References

[1] Newman et al. (2005) Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Cryst. D 61:1426.