Dibenzylcyclooctyne-PEG4-FLAGtag (DYKDDDDK)
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CLK-033-S | 0,5 μmol | 197,60 | Add to Basket/Quote Add to Notepad |
| CLK-033-L | 5 x 0,5 μmol | 793,50 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C80H107N15O30S
Molecular Weight: 1790.85 g/mol
Exact Mass: 1789.70 g/mol
Purity: ≥ 95 % (HPLC)
Form: solid
Color: white to off-white
Solubility: water, PBS (up to 30 mM tested)
Spectroscopic Properties: λmax 308 nm, ε 13.8.0 L mmol-1 cm-1
Description:
DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.
DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 μM)[1] or labeling of Azide-functionalized micelles (amount: 100 nmol)[2]. These concentrations may serve as a starting point for individual assay set-up.
Related products: Azide-PEG3-FLAG, #CLK-032
BIOZ Product Citations:
Selected References:
[1] Patterson et al. (2014) Improved cyclopropene reporters for probing protein glycosylation. Molecular BioSystems 10:1693.
[2] Chan et al. (2013) Double Click: Dual Functionalized Polymeric Micelles with Antibodies and Peptides. Bioconjugate Chemistry 24:105.
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