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DBCO-PEG4-FLAG

Dibenzylcyclooctyne-PEG4-FLAGtag (DYKDDDDK)

Cat. No. Amount Price (EUR) Buy / Note
CLK-033-S 0,5 μmol 161,00 Add to Basket/Quote Add to Notepad
CLK-033-L 5 x 0,5 μmol 646,90 Add to Basket/Quote Add to Notepad
Structural formula of DBCO-PEG4-FLAG
Structural formula of DBCO-PEG4-FLAG

For research use only!

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C80H107N15O30S

Molecular Weight: 1790.85 g/mol

Exact Mass: 1789.70 g/mol

Purity: ≥ 95 % (HPLC)

Form: solid

Color: white to off-white

Solubility: water, PBS (up to 30 mM tested)

Spectroscopic Properties: λmax 308 nm, ε 13.8.0 L mmol-1 cm-1

Description:
DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.

DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 μM)[1] or labeling of Azide-functionalized micelles (amount: 100 nmol)[2]. These concentrations may serve as a starting point for individual assay set-up.

Related products: Azide-PEG3-FLAG, #CLK-032

Selected References:
[1] Patterson et al. (2014) Improved cyclopropene reporters for probing protein glycosylation. Molecular BioSystems 10:1693.
[2] Chan et al. (2013) Double Click: Dual Functionalized Polymeric Micelles with Antibodies and Peptides. Bioconjugate Chemistry 24:105.