For research use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C80H107N15O30S
Molecular Weight: 1790.85 g/mol
Exact Mass: 1789.70 g/mol
Purity: ≥ 95 % (HPLC)
Color: white to off-white
Solubility: water, PBS (up to 30 mM tested)
Spectroscopic Properties: λmax 308 nm, ε 13.8.0 L mmol-1 cm-1
DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC). The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.
DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 μM) or labeling of Azide-functionalized micelles (amount: 100 nmol). These concentrations may serve as a starting point for individual assay set-up.
Related products: Azide-PEG3-FLAG, #CLK-032
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
 Patterson et al. (2014) Improved cyclopropene reporters for probing protein glycosylation. Molecular BioSystems 10:1693.
 Chan et al. (2013) Double Click: Dual Functionalized Polymeric Micelles with Antibodies and Peptides. Bioconjugate Chemistry 24:105.