Azide-PEG3-FLAGtag (DYKDDDDK)
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| CLK-032-S | 0,5 μmol | 197,60 | Add to Basket/Quote Add to Notepad |
| CLK-032-L | 5 x 0,5 μmol | 793,50 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C59H88N16O27S
Molecular Weight: 1485.49 g/mol
Exact Mass: 1484.57 g/mol
Purity: ≥ 95 % (HPLC)
Form: solid
Color: white to off-white
Solubility: water, PBS (up to 30 mM tested)
Description:
Azide-PEG3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne- or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively.
The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.
Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 μM)[1]. This concentrations may serve as a starting point for individual assay set-up.
Related products: DBCO-PEG4-FLAG, #CLK-033
BIOZ Product Citations:
Selected References:
[1] Wang et al. (2011) Sulfated Ligands for the Copper (I)-catalyzed Azide-Alkyne Cycloaddition. Chem Asian J. 6 (10):2796.
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