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Azide-PEG3-FLAG

Azide-PEG3-FLAGtag (DYKDDDDK)

Cat. No. Amount Price (EUR) Buy / Note
CLK-032-S 0,5 μmol 158,20 Add to Basket/Quote Add to Notepad
CLK-032-L 5 x 0,5 μmol 635,50 Add to Basket/Quote Add to Notepad
Structural formula of Azide-PEG3-FLAG
Structural formula of Azide-PEG3-FLAG

For research use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C59H88N16O27S

Molecular Weight: 1485.49 g/mol

Exact Mass: 1484.57 g/mol

Purity: ≥ 95 % (HPLC)

Form: solid

Solubility: water, PBS (up to 30 mM tested)

Description:
Azide-PEG3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne- or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively.

The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.

Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 μM)[1]. This concentrations may serve as a starting point for individual assay set-up.

Related products: DBCO-PEG4-FLAG, #CLK-033

Product Citations:
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.

Selected References:
[1] Wang et al. (2011) Sulfated Ligands for the Copper (I)-catalyzed Azide-Alkyne Cycloaddition. Chem Asian J. 6 (10):2796.