For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C59H88N16O27S
Molecular Weight: 1485.49 g/mol
Exact Mass: 1484.57 g/mol
Purity: ≥ 95 % (HPLC)
Solubility: water, PBS (up to 30 mM tested)
Azide-PEG3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne- or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively.
The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker.
Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 μM). This concentrations may serve as a starting point for individual assay set-up.
Related products: DBCO-PEG4-FLAG, #CLK-033
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
 Wang et al. (2011) Sulfated Ligands for the Copper (I)-catalyzed Azide-Alkyne Cycloaddition. Chem Asian J. 6 (10):2796.