5-Ethynyl-2'-deoxyuridine 5'-triphosphate, Sodium salt
For research use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H15N2O14P3 (free acid)
Molecular Weight: 492.16 g/mol (free acid)
Purity: ≥ 95 %
Form: clear aqueous solution
Concentration: 100 mM - 110 mM
Spectroscopic Properties: λmax 288 nm; ε 12.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Vent exo- or KOD XL)[1,2]
Incorporation into DNA by Primer Extension with family A polymerase Taq and family B polymerases (Pwo, Deep Vent exo- or KOD XL)
The resulting ethynyl-functionalized DNA can subsequently be processed via Cu(I)-catalyzed click chemistry that offers the choice
 Gierlich et al. (2007) Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry. Chem. Eur. J. 13:9486.
 Burley et al. (2006) Directed DNA Metallization. J. Am. Chem. Soc. 128 (5):1398.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.