For research use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
store dry and under inert gas
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C11H13N3O4
Molecular Weight: 251.24 g/mol
Exact Mass: 251.09 g/mol
Purity: ≥ 99 % (HPLC)
Spectroscopic Properties: λmax 291 nm, ε 8.5 L mmol-1 cm-1 (Tris-HCl pH 7.5)
DNA synthesis monitoring
Ethynyl-labeled deoxycytidine (5-EdC) can be used as a replacement for BrdU (5-Bromo-2’-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdC is cell-permeabel and incorporates into replicating DNA instead of its natural analog thymidine.
The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to
introduce a Biotin group (Azides of Biotin) for subsequent purification
tasks or a fluorescent group (Azides of fluorescent dyes) for subsequent
microscopic imaging .
Presolski et al. and Hong et al. provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
 Guan et al. (2011) Intracellular detection of cytosine incorporation in genomic DNA by using 5-ethynyl-2'-deoxycytidine. ChemBioChem 2 (14):2184.
 Presolski et al. (2011) Copper-Catalyzed Azide-Alkyne Click Chemistry for Bioconjugation. Current Protocols in Chemical Biology 3:153.
 Hong et al. (2011) Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation. Angew. Chem. Int. Ed. 48:9879.