Enzymatic CLICK-functionalization of DNA

Nucleotide Selector for DNA Labeling
Select a suitable nucleotide substrate for enzymatic DNA Labeling.

Table 1: Enzymatic incorporation of CLICK-functionalized nucleotides.

PCR: Polymerase chain reaction with Taq Polymerase (CLK-T09, NU-1705) or family B polymerases Pwo, Deep Vent exo^- and KOD XL (CLK-T05/-T06/-T07); NT: Nick Translation with DNAse I / DNA Polymerase I;
Primer Extension: Primer Extension with family B polymerases Pwo, Deep Vent exo^- and KOD XL; 3'-End Labeling: Incorporation with Terminal deoxynucleotidyl Transferase (TdT); RT:: Reverse Transcription with Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT)

		DNA Labeling				cDNA Labeling
Nucleotide	Cat. No.	PCR	NT	Primer Extension	3’ End Labeling	RT
dUTP
5-DBCO-PEG4-dUTP	CLK-048	✔¹	n/a	n/a	n/a	n/a
5-Azidomethyl-dUTP	CLK-084	n/a	n/a	n/a	n/a	n/a
Azide-PEG₄-Aminoallyl-dUTP	NU-1705	✔¹	n/a	n/a	✔²	n/a
5-Ethynyl-dUTP	CLK-T07	✔^1,2	n/a	✔²	n/a	n/a
C8-Alkyne-dUTP	CLK-T05	✔^1,2	n/a	✔²	n/a	n/a
5-TCO-PEG₄-dUTP	CLK-035	✔¹	n/a	n/a	n/a	n/a
5-Vinyl-dUTP	CLK-068	✔¹	n/a	n/a	n/a	n/a
dCTP
C8-Alkyne-dCTP	CLK-T06	✔^1,2	n/a	✔²	✔²	n/a
5-DBCO-PEG₄-dCTP	CLK-060	✔¹	n/a	n/a	n/a	n/a
5-Azido-PEG₄-dCTP	CLK-070	✔¹	n/a	n/a	n/a	n/a
dATP
N⁶-Azidohexyl-dATP	CLK-NU-002	✔¹	n/a	n/a	n/a	n/a